Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen, China.
Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China.
Obesity (Silver Spring). 2024 Mar;32(3):571-582. doi: 10.1002/oby.23964. Epub 2023 Dec 19.
The aim of this study was to identify the differential metabolic characteristics of children with overweight and obesity and understand their potential mechanism in different age stratifications.
Four hundred seventy-three children were recruited and divided into two age stratifications: >4 years (older children) and ≤4 years (younger children), and overweight and obesity were defined according to their BMI percentile. A one dimensional proton nuclear magnetic resonance ( H-NMR)-based metabolomics strategy combined with pattern recognition methods was used to identify the metabolic characteristics of childhood overweight and obesity.
Four and sixteen potential biomarkers related to overweight and two and twenty potential biomarkers related to obesity were identified from younger and older children, respectively. Fluctuations in phenylalanine, tyrosine, glutamine, leucine, histidine, and ascorbate co-occurred in children with obesity at two age stratifications. The disturbances in biosynthesis and metabolism of amino acids, lipid metabolism, and galactose metabolism disturbance were mainly involved in children with overweight and obesity.
The metabolic disturbances show a significant progression from overweight to obesity in children, and different metabolic characteristics were demonstrated in age stratifications. The changes in the levels of phenylalanine, tyrosine, glutamine, leucine, histidine, and ascorbate were tracked with the persistence of childhood obesity. These findings will promote the mechanistic understanding of childhood overweight and obesity.
本研究旨在确定超重和肥胖儿童的差异代谢特征，并了解其在不同年龄分层中的潜在机制。
招募了 473 名儿童，并将其分为两个年龄分层：>4 岁（大年龄儿童）和≤4 岁（小年龄儿童），根据 BMI 百分位数定义超重和肥胖。采用基于一维质子核磁共振（1H-NMR）的代谢组学策略结合模式识别方法，鉴定儿童超重和肥胖的代谢特征。
从小年龄和大年龄儿童中分别鉴定出与超重相关的 4 个和 16 个潜在生物标志物，与肥胖相关的 2 个和 20 个潜在生物标志物。肥胖儿童在两个年龄分层中均出现苯丙氨酸、酪氨酸、谷氨酰胺、亮氨酸、组氨酸和抗坏血酸的波动。氨基酸、脂质代谢和半乳糖代谢紊乱的生物合成和代谢紊乱主要与超重和肥胖儿童有关。
代谢紊乱在儿童中从超重向肥胖显著进展，并且在年龄分层中表现出不同的代谢特征。苯丙氨酸、酪氨酸、谷氨酰胺、亮氨酸、组氨酸和抗坏血酸水平的变化与儿童肥胖的持续存在有关。这些发现将促进对儿童超重和肥胖的机制理解。
