[用于测定碳水化合物代谢戊糖磷酸途径中脱氢酶活性的极谱法]
[Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism].
作者信息
Makarov S A, Kudriavtseva G V, Shishkin V I
出版信息
Vopr Med Khim. 1986 Nov-Dec;32(6):126-8.
PMID:3811277
Abstract
A procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. The procedure involved polarographic registration of oxygen consumption, which is directly related to content of NADPH formed, in a coupled enzymatic system containing peroxidase and Mn2+.
摘要
已开发出一种用于测定人红细胞中葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶活性的方法。该方法涉及在含有过氧化物酶和Mn2+的偶联酶系统中，通过极谱法记录耗氧量，而耗氧量与形成的NADPH含量直接相关。
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[Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism].[用于测定碳水化合物代谢戊糖磷酸途径中脱氢酶活性的极谱法]
Vopr Med Khim. 1986 Nov-Dec;32(6):126-8.
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