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[一种改良的高灵敏度分光光度法测定动物肝脏中的酸性磷酸酶活性]

[A modified highly sensitive spectrophotometric method for determining acid phosphatase activity in the animal liver].

作者信息

Siatkin S P, Frolov V A

出版信息

Vopr Med Khim. 1986 Nov-Dec;32(6):132-4.

PMID:3811279
Abstract

A modified spectrophotometric procedure is described for estimation of acid phosphatase activity in rabbit liver tissue. Coefficient of instrumental sensitivity (SA/C = 0.029), apparent molar absorption (epsilon 820 = 30,500), relative sensitivity of measurement (Cmin = 0.18 mM) were calculated as well as main metrological patterns were evaluated, which showed that the procedure was highly sensitive, accurate and reproducible. Standard error did not exceed 1% and standard deviation was not above 5%.

摘要

描述了一种用于测定兔肝组织中酸性磷酸酶活性的改良分光光度法。计算了仪器灵敏度系数(SA/C = 0.029)、表观摩尔吸光率(ε820 = 30500)、测量相对灵敏度(Cmin = 0.18 mM),并评估了主要计量模式,结果表明该方法具有高灵敏度、准确性和可重复性。标准误差不超过1%,标准差不高于5%。

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