Department of Dental Implantology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing 210029, China.
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing 210029, China.
J Proteome Res. 2024 Jan 5;23(1):344-355. doi: 10.1021/acs.jproteome.3c00588. Epub 2023 Dec 19.
Diabetes mellitus hinders the process of bone regeneration by inhibiting the function of mesenchymal stem cells (MSCs) through elevated glucose levels, thereby impeding osteointegration. The stem cell niche (SCN) plays a crucial role in determining the fate of stem cells by integrating various signals. However, the precise mechanism by which high glucose levels affect the SCN and subsequently influence the function of MSCs remains unclear. In this study, we employed proteomic analysis to identify proteins with altered expression in the extracellular matrix (ECM), aiming to elucidate the underlying mechanism. Three cell supernatants were collected from bone marrow mesenchymal stem cells (BMSCs) or BMSCs stimulated with high glucose (BMSCs+Hg). A total of 590 differentially expressed proteins were identified, which were found to be associated with the ECM, including aging, autophagy, and osteogenic differentiation. The findings of our study indicate that elevated glucose levels exert an influence on the molecular aspects of the SCN, potentially contributing to a better comprehension of the underlying mechanism.
糖尿病通过升高的葡萄糖水平抑制间充质干细胞（MSCs）的功能，从而阻碍骨整合，阻碍骨再生。干细胞龛（SCN）通过整合各种信号，在决定干细胞命运方面起着关键作用。然而，高葡萄糖水平如何影响 SCN 以及随后影响 MSCs 功能的确切机制尚不清楚。在这项研究中，我们采用蛋白质组学分析来鉴定细胞外基质（ECM）中表达改变的蛋白质，旨在阐明潜在的机制。从骨髓间充质干细胞（BMSCs）或高葡萄糖刺激的 BMSCs（BMSCs+Hg）中收集了 3 种细胞上清液。共鉴定出 590 种差异表达蛋白，这些蛋白与 ECM 有关，包括衰老、自噬和成骨分化。本研究的结果表明，升高的葡萄糖水平对 SCN 的分子方面产生影响，可能有助于更好地理解潜在机制。
