Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China; School of Medicine, Shanxi Datong University, Shanxi, Datong 037009, PR China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Food Chem. 2024 May 15;440:138190. doi: 10.1016/j.foodchem.2023.138190. Epub 2023 Dec 10.
Java tea (Clerodendranthus spicatus) has been favored for its various health benefits and abundance of phenolic substances. Steam explosion (SE) treatment was performed in the pretreatment of Java tea stems and the physical properties, phenolic profile and antioxidant capacity were investigated. Extraction kinetics study showed that the phenolics yields of Java tea stems treated at 2.4 MPa for 10 min reached the maximum in 40 min, which was approximately 3 times the yields of raw stems in 180 min. The antioxidant activities of the extracts of Java tea stems were also significantly increased after SE treatment (P < 0.05). In addition, 19 phenolics were detected in Java tea stems by HPLC/QTOF-MS/MS, and rosmarinic acid was found to be hydrolyzed to danshensu during the SE process. SE could be an efficient pretreatment technology to improve the extraction rates of phenolics and conversions of their high-value hydrolyzed products, which could facilitate further research of Java tea products.
辣木叶具有多种保健功效，且富含酚类物质，因此备受青睐。本研究采用蒸汽爆破（SE）预处理辣木叶茎，并考察了 SE 处理前后辣木叶茎的物理特性、酚类物质组成和抗氧化能力。结果表明，在 2.4 MPa 下处理 10 min 可使辣木叶茎的提取得率在 40 min 内达到最大值，约为未处理原料在 180 min 提取得率的 3 倍。SE 处理后，辣木叶茎提取物的抗氧化活性也显著提高（P<0.05）。此外，采用高效液相色谱-四极杆飞行时间质谱联用技术（HPLC/QTOF-MS/MS）共检测到辣木叶茎中的 19 种酚类物质，发现 SE 过程中天麻素发生水解生成丹酚酸。因此，SE 可作为一种有效的预处理技术，提高酚类物质的提取率，并促进其高值水解产物的转化，为辣木叶产品的进一步研究提供参考。
