Pongprayoon Prapasiri, Kuntip Nattapon, Suwanasopee Thanathip, Jattawa Danai, Niramitranon Jitti, Japrung Deanpen, Koonawootrittriron Skorn
Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.
J Biomol Struct Dyn. 2025 Apr;43(6):2772-2780. doi: 10.1080/07391102.2023.2294378. Epub 2023 Dec 20.
Serum albumin (SA) is the most prevalent protein found in blood. Human albumin was used as an albumin substitute in hypoalbuminemia pets due to high sequence similarity. SAs from furry animals were also reported to be the major indoor allergens. Sensitizing to one of SAs coupled with high sequence identity can lead to cross-reactive antibodies in allergic individuals. Thus, understanding the structural and dynamic characters of SAs is crucial for not only albumin substitution but also allergen therapy. Herein, Molecular dynamics (MD) simulations were performed to elucidate the structural and dynamic dissimilarity and similarity of economic animals [equine (ESA), caprine (CASA), ovine (OSA), and leporine (LSA)] to albumins from human (HSA), bovine (BSA), porcine (PSA), and pets [cat (FSA) and dog (CSA)]. The aim is to explore the feasibility of HSA substitution and understand how albumins cause the cross-reactivity. Generally, all albumins studied here show the scissoring motion like other mammalian albumins. The uniqueness of each albumin is defined by different sequence identity of domain I. Also, the drug binding affinity of studied albumins differs from HSA, CSA, FSA, BSA, and PSA. Especially, LSA displays the most deviated behavior from the group. So, such albumin may not be suitable for albumin therapy for pets and humans. CASA, OSA, and ESA share similar characteristics, therefore it is possible to use them to monitor the osmotic pressure among their species, but the allergenic response must be seriously considered. An insight obtained here can be useful to develop albumin therapy and understand clinical allergy.
血清白蛋白（SA）是血液中最普遍存在的蛋白质。由于序列相似度高，人白蛋白被用作低白蛋白血症宠物的白蛋白替代品。有报道称，来自毛皮动物的SA也是主要的室内过敏原。对其中一种SA致敏并伴有高序列同一性，可能会在过敏个体中引发交叉反应性抗体。因此，了解SA的结构和动态特征不仅对白蛋白替代至关重要，对过敏原治疗也至关重要。在此，进行了分子动力学（MD）模拟，以阐明经济动物（马（ESA）、山羊（CASA）、绵羊（OSA）和兔（LSA））与人（HSA）、牛（BSA）、猪（PSA）以及宠物（猫（FSA）和狗（CSA））白蛋白的结构和动态差异与相似性。目的是探索HSA替代的可行性，并了解白蛋白如何引起交叉反应性。一般来说，这里研究的所有白蛋白都表现出与其他哺乳动物白蛋白类似的剪刀状运动。每种白蛋白的独特性由结构域I的不同序列同一性定义。此外，所研究白蛋白的药物结合亲和力与HSA、CSA、FSA、BSA和PSA不同。特别是，LSA表现出与该组最偏离的行为。因此，这种白蛋白可能不适合用于宠物和人类的白蛋白治疗。CASA、OSA和ESA具有相似的特征，因此有可能用它们来监测其物种间的渗透压，但必须认真考虑过敏反应。此处获得的见解可能有助于开发白蛋白治疗方法并理解临床过敏情况。
