AstraZeneca Computational Pathology GmbH, Deutschland.
Department of Pathology, Instituto Nacional de Cancerología (Mexico), Mexico.
Ultrastruct Pathol. 2024 Jan 2;48(1):75-79. doi: 10.1080/01913123.2023.2292559. Epub 2024 Jan 9.
Since first described, several studies about Myxoinflammatory fibroblastic sarcomas (MIFS) have been published stating the clinicopathological, morphological and immunohistochemical features. However, the ultrastructural findings of these MIFS are limited. Thus, the objective of the present paper is to describe the ultrastructural characteristics of these type of tumors by utilizing tissue that was embedded in paraffin and submitted for immunohistochemistry.The tissue of seven different cases was obtained for ultrastructural study with automatized staining devices, that were later observed by using transmission electron microscopy. Histologically all cases displayed conventional structures of Myxoinflammatory fibroblastic sarcoma (Reed-Sternberg like cells, pseudolipoblasts and emperipolesis). Conversely, two of them exhibited high-grade components, one rich in osteoclastic type giant cells and hypercellular areas, and another one rich in inflammation (Hodgkin-like).After immunohistochemistry, all the samples revealed positivity for CD68 with six cases CD163 and five being positive to CD34, Cyclin-D1, and D2-40. Ultrastructural findings indicated rough endoplasmic reticulum with dilatation of the cisterns that indented the nuclei ("soccer ball" cells), abundant lysosomes, phagolysosomes, and intermediate filaments evidencing this entity as a morphologic continuum that exhibited modified fibroblastic phenotype and variable proportion of macrophagic differentiation.
自首次描述以来，已经有几项关于黏液炎症性纤维母细胞肉瘤（MIFS）的研究发表，阐述了其临床病理、形态学和免疫组织化学特征。然而，这些 MIFS 的超微结构发现有限。因此，本研究的目的是利用经石蜡包埋并进行免疫组织化学染色的组织来描述这些肿瘤的超微结构特征。对 7 例不同病例的组织进行超微结构研究，采用自动化染色设备，随后通过透射电子显微镜观察。组织学上，所有病例均显示出典型的黏液炎症性纤维母细胞肉瘤结构（Reed-Sternberg 样细胞、假脂肪母细胞和 emperipolesis）。然而，其中 2 例具有高级别成分，1 例富含破骨细胞样巨细胞和细胞丰富区，另 1 例富含炎症（霍奇金样）。免疫组织化学染色后，所有样本均对 CD68 呈阳性，6 例对 CD163 呈阳性，5 例对 CD34、Cyclin-D1 和 D2-40 呈阳性。超微结构发现表明内质网粗糙，池扩张，使核凹陷（“足球”细胞），富含溶酶体、吞噬溶酶体和中间丝，表明该实体是一种形态连续体，表现出纤维母细胞表型的改变和巨噬细胞分化的比例可变。
