Knizner Kevan T, Eisenberg Seth M, Muddiman David C
FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
J Mass Spectrom. 2024 Jan;59(1):e4995. doi: 10.1002/jms.4995.
Novel mass spectrometry (MS) based analytical platforms have enabled scientists to detect and quantify molecules within biological and environmental samples more accurately. Novel MS instrumentation starts as a prototype and, after years of development, can become a commercial product to be used by the larger MS community. Without the initial prototype, many MS-based instruments today would not be produced. Additionally, biotechnology companies are the main drivers for research, development, and production of novel instruments, but the tools for prototyping instrumentation have never been more accessible. Here, we present a tutorial on prototyping instrumentation through the case study of developing the Next Generation IR-MALDESI source to show that an engineering degree is not required to design and construct a prototype instrument with modern hardware and software. We discuss the prototyping process, the necessary skills required for efficient prototyping, and information about common hardware and software used within initial prototypes.
基于新型质谱（MS）的分析平台使科学家能够更准确地检测和量化生物及环境样品中的分子。新型质谱仪器最初是作为原型出现的，经过数年的研发，可成为供广大质谱领域使用的商业产品。如果没有最初的原型，如今许多基于质谱的仪器将无法生产出来。此外，生物技术公司是新型仪器研发和生产的主要驱动力，但用于仪器原型制作的工具从未像现在这样容易获得。在此，我们通过开发下一代红外基质辅助激光解吸/电离源的案例研究，给出一份关于仪器原型制作的教程，以表明设计和构建一台配备现代硬件和软件的原型仪器并不需要工程学位。我们讨论了原型制作过程、高效原型制作所需的必要技能，以及有关初始原型中常用硬件和软件的信息。
