Toulet V, Saux M C, Rouquette C, Penouil F, Brachet-Liermain A
Ann Biol Clin (Paris). 1986;44(5):517-21.
The purpose of this paper is to propose a method for the determination of cyclosporin in whole blood by means of HPLC with UV detection. After a one step extraction into ether, the organic layer is washed NaOH and evaporated to dryness. The residue is reconstituted in a mixture of acetonitrile and water (40/60) (V/V) and injected into the chromatograph. The C8 reversed phase column is warming at 75 degrees C temperature. The mobile phase consists of acetonitrile and water (60/40) (V/V) and the wavelength is set at 210 nm. The technique is rapid, sensitive, reproducible and well suited for therapeutic monitoring and for pharmacokinetic studies.
本文的目的是提出一种采用高效液相色谱-紫外检测法测定全血中环孢素的方法。经一步萃取至乙醚后，有机层用氢氧化钠洗涤并蒸发至干。残留物用乙腈和水（40/60）（V/V）的混合溶液复溶后注入色谱仪。C8反相柱在75℃温度下加热。流动相由乙腈和水（60/40）（V/V）组成，波长设定为210nm。该技术快速、灵敏、可重现，非常适合治疗药物监测和药代动力学研究。
