Chiang T M, Kang E S, Kang A H
Arch Biochem Biophys. 1987 Jan;252(1):15-23. doi: 10.1016/0003-9861(87)90003-8.
Platelets exposed to collagen sufficient to stimulate the release reaction show an increase in labeling of two intracellular proteins with molecular weights of 20,000 and 42,000. The 20,000 Mr protein has already been identified as the light chain of myosin whereas the identity of the 42,000 Mr protein had not been established. By use of biochemical and immunological techniques, the identify of the 42,000 Mr component of prelabeled platelets found in the 100,000g supernatant of freeze-thawed or sonicated cells appears to be one of the subunits of pyruvate dehydrogenase complex which is translocated from the mitochondria to the 100,000g supernatant during the preparative procedure. Increased phosphorylation of the 42,000 Mr protein occurred after collagen stimulation and was accompanied by diminished pyruvate dehydrogenase activity.
暴露于足以刺激释放反应的胶原蛋白的血小板，其两种分子量分别为20,000和42,000的细胞内蛋白质的标记增加。20,000 Mr的蛋白质已被鉴定为肌球蛋白的轻链，而42,000 Mr蛋白质的身份尚未确定。通过生化和免疫技术，在冻融或超声处理细胞的100,000g上清液中发现的预标记血小板的42,000 Mr成分的身份似乎是丙酮酸脱氢酶复合物的亚基之一，该亚基在制备过程中从线粒体转移到100,000g上清液中。胶原蛋白刺激后，42,000 Mr蛋白质的磷酸化增加，并伴有丙酮酸脱氢酶活性降低。
