Chantiri Mansour, Nammour Samir, El Toum Sami, Zeinoun Toni
Department of Periodontology, Faculty of Dental Medicine, Lebanese University, Beirut 27798, Lebanon.
Department of Dental Sciences, Faculty of Medicine, University of Liege, 4000 Liege, Belgium.
Life (Basel). 2023 Dec 4;13(12):2298. doi: 10.3390/life13122298.
The aim of this study is to evaluate the effect on the initiation of new blood vessel formation of rh-BMP-2 administration in the human gingival tissue during bone regeneration surgery.
The randomized controlled clinical trial included twenty patients with bilateral partial edentulous of the mandibular premolar and molar region. Each patient received one implants on each side. Only one side received a 0.25 µg injection of rhBMP-2 into the gingival flap and grafted material during guided bone regeneration (GBR) for dental implantation. And the other side received GBR without injection. Three samples were collected from each patient as follows: one from the anterior area of the mandible (control group #1) collected at the time of all implant surgeries, and the two other samples during the placement of healing abutments at 4 months of follow-up, from treated side with rh-BMP-2 (test group) and untreated ones (control group #2). A total of 60 gingival samples were collected. Samples were stained with hematoxylin-eosin, and immunohistochemistry was performed with a vascular endothelial growth factor marker. The number of new vessels in each sample was counted.
Statistical analyses showed a significantly higher number of new vessels in the gingival tissue of the test group.
Rh-BMP-2 injections into the gingival flap significantly improved new blood vessel formation.
本研究的目的是评估在骨再生手术期间，rh-BMP-2注射入人牙龈组织对新血管形成起始的影响。
这项随机对照临床试验纳入了20例双侧下颌前磨牙和磨牙区部分牙列缺失的患者。每位患者每侧植入一枚种植体。仅一侧在引导骨再生（GBR）植入牙种植体时，于牙龈瓣和移植材料中注射0.25μg rhBMP-2。另一侧接受未注射的GBR。从每位患者收集三个样本，如下：一个来自下颌前部区域（对照组#1），在所有种植手术时采集；另外两个样本在随访4个月放置愈合基台时采集，分别来自注射rhBMP-2的治疗侧（试验组）和未治疗侧（对照组#2）。共收集60个牙龈样本。样本用苏木精-伊红染色，并使用血管内皮生长因子标记物进行免疫组织化学检测。对每个样本中的新血管数量进行计数。
统计分析显示试验组牙龈组织中的新血管数量显著更多。
向牙龈瓣注射rh-BMP-2可显著改善新血管形成。
