通过粉末冶金路线获得的无镍奥氏体不锈钢的生物活性、细胞毒性和摩擦学研究。

Bioactivity, Cytotoxicity, and Tribological Studies of Nickel-Free Austenitic Stainless Steel Obtained via Powder Metallurgy Route.

作者信息

Romanczuk-Ruszuk Eliza, Krawczyńska Agnieszka, Łukaszewicz Andrzej, Józwik Jerzy, Tofil Arkadiusz, Oksiuta Zbigniew

机构信息

Institute of Biomedical Engineering, Faculty of Mechanical Engineering, Bialystok University of Technology, Wiejska 45C, 15-351 Bialystok, Poland.

Faculty of Materials Science and Engineering, Warsaw University of Technology, Wołoska 141, 02-507 Warsaw, Poland.

出版信息

Materials (Basel). 2023 Dec 14;16(24):7637. doi: 10.3390/ma16247637.

DOI:10.3390/ma16247637

PMID:38138779

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10744826/

Abstract

In the present study, the bioactivity, cytotoxicity, and tribological properties of a nickel-free austenitic stainless steel produced via the mechanical alloying of elemental iron, chromium, and manganese nitride powders following by hot isostatic pressing was investigated. Powders after 90 h of mechanical alloying were consolidated via hot isostatic pressing at 1150 °C (1425 K) and heat treated at 1175 °C (1448 K) for 1 h in a vacuum with furnace cooling. Tribological tests were performed to determine the resistance of the as-received nickel-free steel. It was noticed that applying heat treatment after hot isostatic pressing decreases the average friction coefficient and wear rate of the austenitic steel. An immersion test in a simulated body fluid for 28 days at 37 ± 1 °C has been used to determine the biocompatibility of the tested material. The SEM-EDS analysis allowed us to characterise the morphology of the films and the elements of the steel on the thin-film layer. Elements typical of apatite (calcium and phosphorus) were detected on the surface of the sample. Cellular toxicity tests showed no significant toxic side effects for Saos-2 human osteosarcoma cells and the number of Saos-2 human osteosarcoma cells on the nickel-free steel was greater than on the 316LV grade steel.

摘要

在本研究中，对通过将元素铁、铬和氮化锰粉末进行机械合金化后再进行热等静压制备的无镍奥氏体不锈钢的生物活性、细胞毒性和摩擦学性能进行了研究。经过90小时机械合金化后的粉末在1150°C（1425K）下通过热等静压进行固结，并在真空中于1175°C（1448K）下进行1小时的热处理，随后炉冷。进行摩擦学测试以确定所接收的无镍钢的耐磨性。结果发现，在热等静压后进行热处理可降低奥氏体钢的平均摩擦系数和磨损率。在37±1°C的模拟体液中进行28天的浸泡试验，以确定测试材料的生物相容性。扫描电子显微镜-能谱分析使我们能够表征薄膜的形态以及薄膜层上钢的元素。在样品表面检测到了磷灰石典型的元素（钙和磷）。细胞毒性测试表明，对于Saos-2人骨肉瘤细胞没有明显的毒副作用，并且无镍钢上的Saos-2人骨肉瘤细胞数量多于316LV级钢上的细胞数量。