Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin 8, Saint Petersburg 196608, Russia.
Int J Mol Sci. 2023 Dec 5;24(24):17144. doi: 10.3390/ijms242417144.
Despite global warming, the influence of heat on symbiotic nodules is scarcely studied. In this study, the effects of heat stress on the functioning of nodules formed by bv. strain 3841 on pea () line SGE were analyzed. The influence of elevated temperature was analyzed at histological, ultrastructural, and transcriptional levels. As a result, an unusual apical pattern of nodule senescence was revealed. After five days of exposure, a senescence zone with degraded symbiotic structures was formed in place of the distal nitrogen fixation zone. There was downregulation of various genes, including those associated with the assimilation of fixed nitrogen and leghemoglobin. After nine days, the complete destruction of the nodules was demonstrated. It was shown that nodule recovery was possible after exposure to elevated temperature for 3 days but not after 5 days (which coincides with heat wave duration). At the same time, the exposure of plants to optimal temperature during the night leveled the negative effects. Thus, the study of the effects of elevated temperature on symbiotic nodules using a well-studied pea genotype and strain led to the discovery of a novel positional response of the nodule to heat stress.
尽管全球变暖，但对共生结瘤受热量影响的研究甚少。本研究分析了热应激对豌豆（）SGE 品系上 bv. 菌株 3841 形成的结瘤功能的影响。在组织学、超微结构和转录水平上分析了高温的影响。结果揭示了一种不寻常的结瘤顶端衰老模式。暴露五天后，在远端固氮区形成了一个含有降解共生结构的衰老区。各种基因的表达下调，包括与固定氮同化和豆血红蛋白相关的基因。九天后，完全破坏了结瘤。结果表明，暴露于高温 3 天后结瘤可以恢复，但 5 天后（与热浪持续时间一致）则不能恢复。同时，植物在夜间暴露于最佳温度可减轻负面影响。因此，使用经过充分研究的豌豆基因型和菌株研究高温对共生结瘤的影响，发现了结瘤对热应激的一种新的位置响应。
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