Pösö A R, Penttilä K E, Suolinna E M, Lindros K O
Biochem J. 1986 Oct 15;239(2):263-7. doi: 10.1042/bj2390263.
Periportal hepatocytes isolated by digitonin/collagenase perfusion produced urea faster than did similarly prepared perivenous hepatocytes, in both the presence and the absence of amino acids and various urea precursors. There was no difference between the two cell types in rates of intracellular proteolysis. The initial difference in urea synthesis persisted for 5 days during primary culture, but then gradually disappeared. Our results demonstrate that the periportal dominance of urea formation is unrelated to the currently existing acinar microenvironment in the intact liver, but probably reflects differences in acinar key enzyme activities only slowly converging during culture.
通过洋地黄皂苷/胶原酶灌注分离的门周肝细胞，无论有无氨基酸和各种尿素前体，其产生尿素的速度都比同样制备的中央静脉周围肝细胞快。两种细胞类型在细胞内蛋白水解速率上没有差异。尿素合成的初始差异在原代培养期间持续了5天，但随后逐渐消失。我们的结果表明，尿素形成的门周优势与完整肝脏中目前存在的腺泡微环境无关，但可能仅反映了在培养过程中腺泡关键酶活性的差异，且这种差异收敛缓慢。
