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汇管区和中央静脉周围的肝细胞在原代培养中保持其区域特征。

Periportal and perivenous hepatocytes retain their zonal characteristics in primary culture.

作者信息

Quistorff B, Dich J, Grunnet N

出版信息

Biochem Biophys Res Commun. 1986 Sep 30;139(3):1055-61. doi: 10.1016/s0006-291x(86)80284-4.

DOI:10.1016/s0006-291x(86)80284-4
PMID:3021146
Abstract

Periportal and perivenous hepatocytes from rat liver were isolated by combined digitonin-collagenase perfusion, and gluconeogenesis, urea synthesis and fatty acid synthesis was measured both in freshly isolated cells and in primary culture. A periportal zonation of gluconeogenesis and urea synthesis of about 3 and 1.5 fold, respectively, was observed. This zonation persisted unchanged for 23 hours in culture under identical conditions of incubation for periportal and perivenous cells. Fatty acid synthesis was not zonated.

摘要

通过洋地黄皂苷 - 胶原酶联合灌注法分离大鼠肝脏的门周和中央静脉周围肝细胞，并在新鲜分离的细胞和原代培养物中测定糖异生、尿素合成和脂肪酸合成。观察到糖异生和尿素合成的门周区域化分别约为3倍和1.5倍。在门周和中央静脉周围细胞相同的培养条件下，这种区域化在培养23小时内保持不变。脂肪酸合成不存在区域化。

相似文献

1
Periportal and perivenous hepatocytes retain their zonal characteristics in primary culture.汇管区和中央静脉周围的肝细胞在原代培养中保持其区域特征。
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1055-61. doi: 10.1016/s0006-291x(86)80284-4.
2
Gluconeogenesis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin/collagenase perfusion technique.采用一种新的高产洋地黄皂苷/胶原酶灌注技术分离大鼠肝脏门周和肝静脉周围肝细胞中的糖异生作用。
Biochem J. 1985 Jul 1;229(1):221-6. doi: 10.1042/bj2290221.
3
Different capacities for amino acid transport in periportal and perivenous hepatocytes isolated by digitonin/collagenase perfusion.通过洋地黄皂苷/胶原酶灌注分离的门静脉周围和肝静脉周围肝细胞中氨基酸转运的不同能力。
Hepatology. 1989 Jan;9(1):22-8. doi: 10.1002/hep.1840090105.
4
Dual-digitonin-pulse perfusion. Concurrent sampling of periportal and perivenous cytosol of rat liver for determination of metabolites and enzyme activities.双洋地黄皂苷脉冲灌注。同时采集大鼠肝脏门静脉周围和肝静脉周围的胞质溶胶，用于测定代谢物和酶活性。
Biochem J. 1987 Apr 1;243(1):87-95. doi: 10.1042/bj2430087.
5
Urea synthesis in freshly isolated and in cultured periportal and perivenous hepatocytes.新鲜分离的以及培养的门静脉周围和肝静脉周围肝细胞中的尿素合成。
Biochem J. 1986 Oct 15;239(2):263-7. doi: 10.1042/bj2390263.
6
Digitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes.用于高效分离门静脉周围或肝静脉周围肝细胞的洋地黄皂苷-胶原酶灌注法。
Biochem J. 1985 Jun 15;228(3):757-60. doi: 10.1042/bj2280757.
7
Induction in primary culture of 'gluconeogenic' and 'glycolytic' hepatocytes resembling periportal and perivenous cells.在原代培养中诱导出类似门静脉周围和肝静脉周围细胞的“糖异生”和“糖酵解”肝细胞。
Eur J Biochem. 1982 Aug;126(2):271-8. doi: 10.1111/j.1432-1033.1982.tb06775.x.
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Biochemical and morphological studies on perivenous and periportal liver parenchymal cells from female rats isolated by digitonin-collagenase method.采用洋地黄皂苷-胶原酶法分离雌性大鼠肝小叶中央静脉周围和门静脉周围肝实质细胞的生化及形态学研究。
Exp Pathol. 1991;42(3):159-68. doi: 10.1016/s0232-1513(11)80018-6.
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Zonation of hepatic lipogenic enzymes identified by dual-digitonin-pulse perfusion.通过双洋地黄皂苷脉冲灌注鉴定的肝脏脂肪生成酶的区域化
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