牛β-酪蛋白cDNA的克隆与序列分析
Cloning and sequence analysis of bovine beta-casein cDNA.
作者信息
Jimenez-Flores R, Kang Y C, Richardson T
出版信息
Biochem Biophys Res Commun. 1987 Jan 30;142(2):617-21. doi: 10.1016/0006-291x(87)90318-4.
A bovine beta-casein cDNA clone was isolated from a cDNA library prepared from mammary gland mRNA. Sequence analysis revealed 25 nucleotides (nt) of the 5' noncoding region, 672 nt of the complete sequence coding and a 3' region of approximately 500 nt. When the nucleotide sequence of bovine beta-casein cDNA is compared to rat beta-casein cDNA (5), a high degree of homology is observed in the first 100 nt corresponding to the signal peptide of the pre-beta-caseins.
从乳腺mRNA制备的cDNA文库中分离出一个牛β-酪蛋白cDNA克隆。序列分析显示5'非编码区有25个核苷酸（nt），完整编码序列有672 nt，以及大约500 nt的3'区。当将牛β-酪蛋白cDNA的核苷酸序列与大鼠β-酪蛋白cDNA（5）进行比较时，在前100 nt中观察到与前β-酪蛋白信号肽相对应的高度同源性。
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