Dairaku K, Miyagi T, Wakui A, Tsuiki S
Biochem Int. 1986 Nov;13(5):741-8.
Using 4-methylumbelliferyl-N-acetylneuraminic acid as substrate, the cytosolic fractions of various rat tissues were assayed for sialidase activity. The activity was about 16 times greater in skeletal muscle than in liver, and the enzymes from the two sources were identical in chromatographic behavior, pH optimum, and substrate specificity. Apparently the same enzyme was found to be distributed in brain, heart, stomach, intestine, and testis, but not in lung and spleen. Sialidase was also present in kidney cytosol at a high level, but the enzyme resembled liver lysosomal sialidase rather than liver cytosolic sialidase in substrate specificity.
以4-甲基伞形酮基-N-乙酰神经氨酸为底物，对各种大鼠组织的胞质部分进行唾液酸酶活性测定。骨骼肌中的活性比肝脏中的大约高16倍，并且来自这两种来源的酶在色谱行为、最适pH和底物特异性方面是相同的。显然，在脑、心脏、胃、肠和睾丸中发现了相同的酶，但在肺和脾中未发现。肾脏胞质溶胶中也存在高水平的唾液酸酶，但该酶在底物特异性方面类似于肝脏溶酶体唾液酸酶，而不是肝脏胞质溶胶唾液酸酶。
