Liu Jun, Pan Cuili, Yue Hui, Li He, Liu Dunhua, Hu Ziying, Hu Yuanliang, Yu Xiang, Dong Weiwei, Feng Yanli
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
Hubei Engineering Research Center of Special Wild Vegetables Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi 435002, China.
Food Chem X. 2023 Nov 30;20:101038. doi: 10.1016/j.fochx.2023.101038. eCollection 2023 Dec 30.
The study aimed to assess differences in proteomic and metabolite profiles in ageing (1, 2, 4, and 6 days at 4 °C) beef exudates and determine their relationship with beef muscle iron metabolism and oxidation. Proteomic and metabolomic analyses identified 877 metabolites and 1957 proteins. The joint analysis identified 24 differential metabolites (DMs) and 56 differentially expressed proteins (DEPs) involved in 15 shared pathways. Ferroptosis was identified as the only iron metabolic pathway, and 4 DMs (l-glutamic acid, arachidonic acid, glutathione and gamma-glutamylcysteine) and 5 DEPs (ferritin, phospholipid hydroperoxide glutathione peroxidase, heme oxygenase 1, major prion protein, and acyl-CoA synthetase long chain family member 4) were involved in iron metabolism by regulating heme and ferritin degradation, Fe and Fe conversion, arachidonic acid oxidation and inactivation of glutathione peroxidase (GPX) 4, leading to increased levels of free iron, ROS, protein and lipid oxidation ( < 0.05). Overall, abnormal iron metabolism during ageing induced oxidative stress in muscle tissue.
该研究旨在评估老化（4℃下1、2、4和6天）牛肉渗出物中蛋白质组和代谢物谱的差异，并确定它们与牛肉肌肉铁代谢和氧化的关系。蛋白质组学和代谢组学分析鉴定出877种代谢物和1957种蛋白质。联合分析确定了24种差异代谢物（DMs）和56种差异表达蛋白质（DEPs），它们参与15条共享途径。铁死亡被确定为唯一的铁代谢途径，4种DMs（L-谷氨酸、花生四烯酸、谷胱甘肽和γ-谷氨酰半胱氨酸）和5种DEPs（铁蛋白、磷脂氢过氧化物谷胱甘肽过氧化物酶、血红素加氧酶1、主要朊蛋白和酰基辅酶A合成酶长链家族成员4）通过调节血红素和铁蛋白降解、Fe²⁺和Fe³⁺转化、花生四烯酸氧化以及谷胱甘肽过氧化物酶（GPX）4失活参与铁代谢，导致游离铁、活性氧、蛋白质和脂质氧化水平升高（P＜0.05）。总体而言，老化过程中铁代谢异常诱导了肌肉组织中的氧化应激。
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