Liu Jun, Hu Ziying, Ma Qin, Wang Shuzhe, Liu Dunhua
School of Agriculture, Ningxia University, 750021 Yinchuan, China.
School of Food & Wine, Ningxia University, 750021 Yinchuan, China.
Food Chem. 2023 Jun 30;412:135550. doi: 10.1016/j.foodchem.2023.135550. Epub 2023 Jan 24.
Ferroptosis plays a pivotal role in regulating various physiological processes and quality of post-mortem muscle. However, the molecular mechanisms underlying ferroptosis remain unclear. The study investigated how ferroptosis was induced in beef during cold storage. Results showed that the expression of autophagy-related genes, LC3, ATG5, ATG7, and NCOA4 in beef during cold storage promoted the degradation of ferritin heavy chains. Ferritin evoked ferroptosis by releasing free iron, inducing reactive oxygen species (ROS) accumulation and inhibiting the glutathione (GSH)-glutathione peroxidase 4 (GPX4) pathway. Furthermore, treatment of myoblasts with GSK 2656157 (autophagy inhibitor) showed that ferritin degradation was lower in the GSK 2656157-treated myoblasts than in the control, while GSH content and GPX4 activity were higher than the control (P < 0.05), and the contents of free iron, ROS and malondialdehyde, and apoptosis were lower than the control (P < 0.05). These results suggest that ferroptosis is induced by degradation of ferritin via the autophagic pathway.
铁死亡在调节各种生理过程和宰后肌肉品质方面发挥着关键作用。然而，铁死亡背后的分子机制仍不清楚。该研究调查了冷藏期间牛肉中铁死亡是如何诱导的。结果表明，冷藏期间牛肉中自噬相关基因LC3、ATG5、ATG7和NCOA4的表达促进了铁蛋白重链的降解。铁蛋白通过释放游离铁、诱导活性氧（ROS）积累和抑制谷胱甘肽（GSH）-谷胱甘肽过氧化物酶4（GPX4）途径引发铁死亡。此外，用GSK 2656157（自噬抑制剂）处理成肌细胞表明，GSK 2656157处理的成肌细胞中铁蛋白降解低于对照组，而GSH含量和GPX4活性高于对照组（P<0.05），游离铁、ROS和丙二醛含量以及细胞凋亡低于对照组（P<0.05）。这些结果表明，铁死亡是通过自噬途径降解铁蛋白诱导的。
