CRISPR/Cas9 介导的 PTP 表达修饰。

CRISPR/Cas9-Mediated Modification of PTP Expression.

机构信息

Institute for Molecular Cell Biology, CMB - Center for Molecular Biomedicine; University Hospital Jena, Jena, Germany.

Regeneration of Hematopoiesis , Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.

出版信息

Methods Mol Biol. 2024;2743:43-56. doi: 10.1007/978-1-0716-3569-8_3.

DOI:10.1007/978-1-0716-3569-8_3

PMID:38147207

Abstract

Alteration of protein tyrosine phosphatase (PTP) gene expression is a commonly used approach to experimentally analyze their function in the cell physiology of mammalian cells. Here, exemplified for receptor-type PTPRJ (Dep-1, CD148) and PPTRC (CD45), we provide the CRISPR/Cas9-mediated approaches for their inactivation and transcriptional activation using genome editing. These methods are generally applicable to any other protein of interest.

摘要

蛋白质酪氨酸磷酸酶（PTP）基因表达的改变是一种常用的方法，用于在哺乳动物细胞的细胞生理学中实验性地分析它们的功能。在这里，以受体型 PTPRJ（Dep-1，CD148）和 PPTRC（CD45）为例，我们提供了使用基因组编辑的 CRISPR/Cas9 介导的方法来使其失活和转录激活。这些方法通常适用于任何其他感兴趣的蛋白质。