Hospital of Guizhou Panjiang Coal Power Group Co. Ltd, Panzhou, China.
Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
Mol Biotechnol. 2024 May;66(5):1019-1030. doi: 10.1007/s12033-023-01000-w. Epub 2023 Dec 26.
The miR-497-195 cluster facilitates the occurrence and development of cancer. This study aims to investigate whether the miR-195-497 cluster could regulate the progression of colorectal cancer by regulating the common target gene, FOS-related antigen 1 (FRA1). Overexpression of the miR-195/497 vector was used to evaluate the effect of overexpression of miR-195-497 clusters on the biological behavior of colon cancer cells. In animal experiments, tumor growth and metastasis were recorded by constructing a nude mouse model of a subcutaneously implanted tumor. miR-195 and miR-497 were expressed to varying degrees in Caco-2, LoVo, and HT-29 cells. Overexpression of miR-195/497 and inhibition of FRA1 decreased HT-29 cell proliferation, inhibited cell invasion and migration, and promoted Epithelial-mesenchymal transition (EMT). In vivo experiments showed that the overexpression of miR-195/497 or inhibition of FRA1 inhibited tumor growth, affected EMT in tumor cells, and inhibited the expression of FRA1. Additionally, the aforementioned conditions had the best effect when used together. The miR-195-497 cluster can regulate the proliferation, EMT, invasion, and migration of colorectal cancer cells by regulating the common target gene FRA1, thereby affecting the development of colorectal cancer.
miR-497-195 簇促进癌症的发生和发展。本研究旨在探讨 miR-195-497 簇是否可以通过调节共同的靶基因 FOS 相关抗原 1（FRA1）来调节结直肠癌的进展。过表达 miR-195/497 载体用于评估 miR-195-497 簇过表达对结肠癌细胞生物学行为的影响。在动物实验中，通过构建皮下植入肿瘤的裸鼠模型来记录肿瘤生长和转移。Caco-2、LoVo 和 HT-29 细胞中 miR-195 和 miR-497 表达程度不同。miR-195/497 的过表达和 FRA1 的抑制降低了 HT-29 细胞的增殖，抑制了细胞侵袭和迁移，并促进了上皮间质转化（EMT）。体内实验表明，miR-195/497 的过表达或 FRA1 的抑制抑制了肿瘤的生长，影响了肿瘤细胞中的 EMT，并抑制了 FRA1 的表达。此外，当同时使用上述条件时，效果最佳。miR-195-497 簇可以通过调节共同的靶基因 FRA1 来调节结直肠癌细胞的增殖、EMT、侵袭和迁移，从而影响结直肠癌的发展。
