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迈向花粉DNA元条形码技术:法医学的一项突破。

Stepping towards pollen DNA metabarcoding: A breakthrough in forensic sciences.

作者信息

Wasti Qandeel Zaineb, Sabar Muhammad Farooq, Farooq Abeera, Khan Muhammad Umer

机构信息

Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan.

Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan.

出版信息

Forensic Sci Med Pathol. 2024 Dec;20(4):1464-1474. doi: 10.1007/s12024-023-00770-8. Epub 2023 Dec 26.

DOI:10.1007/s12024-023-00770-8
PMID:38147285
Abstract

This review is engaged in determining the capability of plant pollen as a significant source of evidence for the linkage between suspects and crime location in forensic sciences. Research and review articles were collected from Google Scholar, the Web of Science, and PubMed. Articles were searched using specific keywords such as "Forensic Palynology," "Pollen metabarcoding," "Plant forensics," and "Pollen" AND "criminal investigation." Boolean logic was also utilized to narrow the articles to be included in this review article. Through the literature and exploratory research, it has been observed in the current study that with advancements in technology, forensic palynology has found its application in creating an association between the crime scene and suspected individuals to have a link to it, as pollen DNA is a long-lasting investigative tool that can effectively help forensic investigations. Moreover, the literature shows that the DNA of pollen and spores has helped forensic scientists link suspects to crime scenes, and the introduction of pollen DNA metabarcoding tools has eased the efforts of palynologists to analyze pollen DNA. The introduction of DNA metabarcoding techniques to analyze pollen from plants has helped identify the geological locations of the plants and ultimately identify the culprit.

摘要

本综述旨在确定植物花粉作为法医学中嫌疑人与犯罪地点之间联系的重要证据来源的能力。研究和综述文章从谷歌学术、科学网和PubMed收集。使用“法医孢粉学”、“花粉宏条形码分析”、“植物法医学”以及“花粉”和“刑事调查”等特定关键词进行文章搜索。还利用布尔逻辑来缩小纳入本综述文章的范围。通过文献研究和探索性研究,在当前研究中观察到,随着技术的进步,法医孢粉学已在建立犯罪现场与与之有关联的嫌疑人之间的联系方面得到应用,因为花粉DNA是一种持久的调查工具,可以有效地帮助法医调查。此外,文献表明,花粉和孢子的DNA已帮助法医科学家将嫌疑人与犯罪现场联系起来,而花粉DNA宏条形码分析工具的引入减轻了孢粉学家分析花粉DNA的工作量。引入DNA宏条形码分析技术来分析植物花粉有助于确定植物的地理位置,最终识别罪犯。

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本文引用的文献

1
Method for quick DNA barcode reference library construction.快速构建DNA条形码参考文库的方法。
Ecol Evol. 2021 Aug 4;11(17):11627-11638. doi: 10.1002/ece3.7788. eCollection 2021 Sep.
2
Plant biodiversity assessment through pollen DNA metabarcoding in Natura 2000 habitats (Italian Alps).通过花粉 DNA metabarcoding 对 Natura 2000 生境中的植物生物多样性进行评估(意大利阿尔卑斯山)。
Sci Rep. 2021 Sep 14;11(1):18226. doi: 10.1038/s41598-021-97619-3.
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Pollen DNA metabarcoding identifies regional provenance and high plant diversity in Australian honey.
花粉DNA宏条形码技术鉴定澳大利亚蜂蜜的产地来源和植物多样性
Ecol Evol. 2021 Jun 3;11(13):8683-8698. doi: 10.1002/ece3.7679. eCollection 2021 Jul.
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Microfluidic Enrichment Barcoding (MEBarcoding): a new method for high throughput plant DNA barcoding.微流控富集条形码(MEBarcoding):一种高通量植物 DNA 条形码的新方法。
Sci Rep. 2020 May 26;10(1):8701. doi: 10.1038/s41598-020-64919-z.
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Which academic search systems are suitable for systematic reviews or meta-analyses? Evaluating retrieval qualities of Google Scholar, PubMed, and 26 other resources.哪些学术搜索系统适用于系统评价或荟萃分析?评估 Google Scholar、PubMed 和其他 26 个资源的检索质量。
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Plant-pollinator interactions over time: Pollen metabarcoding from bees in a historic collection.植物-传粉者随时间的相互作用:来自历史馆藏中蜜蜂的花粉代谢条形码分析
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Simple detection of bacterioplankton using a loop-mediated isothermal amplification (LAMP) assay: First practical approach to 72 cases of suspected drowning.使用环介导等温扩增(LAMP)分析法简单检测浮游细菌:72例疑似溺水病例的首次实际应用方法
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A protocol for obtaining DNA barcodes from plant and insect fragments isolated from forensic-type soils.一种从法医类型土壤中分离出的植物和昆虫碎片获取DNA条形码的方案。
Int J Legal Med. 2018 Nov;132(6):1515-1526. doi: 10.1007/s00414-018-1772-1. Epub 2018 Feb 8.
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Advancing taxonomy and bioinventories with DNA barcodes.利用DNA条形码推进分类学和生物资源清查。
Philos Trans R Soc Lond B Biol Sci. 2016 Sep 5;371(1702). doi: 10.1098/rstb.2015.0339.
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Pollen DNA barcoding: current applications and future prospects.花粉DNA条形码:当前应用与未来前景
Genome. 2016 Sep;59(9):629-40. doi: 10.1139/gen-2015-0200. Epub 2016 Apr 13.