Division of Medical Laboratory Sciences, Department of Radiological and Medical Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Medical Technology, Faculty of Medical Sciences, Shubun University, Ichinomiya, Japan.
In Vivo. 2024 Jan-Feb;38(1):114-121. doi: 10.21873/invivo.13417.
BACKGROUND/AIM: Our recent studies have indicated that trace copper co-existed with iron in hemosiderin particles of human genetic iron overload. To understand this phenomenon, we analyzed hemosiderin particles in iron-overloaded rat liver by using scanning transmission electron microscopy - energy-dispersive X-ray (STEM-EDX) spectroscopy.
Samples for STEM-EDX spectroscopy were prepared from the liver of rats administered an intraperitoneal injection of dextran iron.
The micro-domain analysis with STEM-EDX spectroscopy showed that dense bodies contained high levels of iron and trace copper. Quantitative analysis of copper levels in the liver specimen using atomic spectrophotometry showed that copper concentration in the liver was not increased by iron overload. These findings suggest that the overload of iron induced distribution of trace copper to hemosiderin particles without changing cellular copper levels.
Co-existence of copper with iron was observed in hemosiderin particles of the liver of an experimental model of iron overload, suggesting that iron overload induced distribution of trace copper into hemosiderin particles.
背景/目的：我们最近的研究表明，在遗传性铁过载患者的含铁血黄素颗粒中，痕量铜与铁共存。为了了解这一现象，我们使用扫描透射电子显微镜-能量色散 X 射线（STEM-EDX）光谱法分析了铁过载大鼠肝脏中的含铁血黄素颗粒。
用腹腔注射葡聚糖铁的方法处理大鼠，制备用于 STEM-EDX 光谱分析的样品。
STEM-EDX 光谱的微区分析显示，致密体内含有高水平的铁和痕量铜。原子分光光度法对肝标本中铜含量的定量分析表明，铁过载并未导致肝内铜浓度升高。这些发现表明，铁过载诱导痕量铜分布到含铁血黄素颗粒中，而不改变细胞内铜水平。
在铁过载实验模型的肝脏含铁血黄素颗粒中观察到铜与铁共存，提示铁过载诱导痕量铜分布到含铁血黄素颗粒中。
