Yang An-Qi, Zheng Wentong, Chen Xiaoyang, Wang Jiayin, Zhou Shuang, Gao Hongzhi
Department of Basic Medicine, Quanzhou Medical College, Quanzhou, China.
Central Laboratory, Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.
Front Bioeng Biotechnol. 2023 Dec 12;11:1256340. doi: 10.3389/fbioe.2023.1256340. eCollection 2023.
In this paper, we demonstrate a surface-enhanced Raman spectroscopy (SERS) biosensor based on the self-assembly of gold nanorods (AuNRs) for the specific detection of airway inflammatory factors in diluted sputum. The AuNR surface was modified with an antibody that was able to specifically recognize an airway inflammatory factor, interleukin-5 (IL-5), so that a end-to-end self-assembly system could be obtained, resulting in an order of magnitude amplification of the Raman signal and greatly improved sensitivity. Meanwhile, the outer layer of the biosensor was coated with silicon dioxide, which improved the stability of the system and facilitated its future applications. When the detected concentration was in the range of 0.1-50 pg/mL, the SERS signal generated by the sensor showed a good linear relationship with the IL-5 concentration. Moreover, it had satisfactory performance in diluted sputum and clinical subjects with asthma, which could achieve sensitive detection of the airway inflammatory factor IL-5. Overall, the developed biosensor based on the SERS effect exhibited the advantages of rapid and sensitive detecting performance, which is suitable for monitoring airway inflammatory factors in sputum.
在本文中，我们展示了一种基于金纳米棒（AuNRs）自组装的表面增强拉曼光谱（SERS）生物传感器，用于特异性检测稀释痰液中的气道炎症因子。用能够特异性识别气道炎症因子白细胞介素-5（IL-5）的抗体修饰金纳米棒表面，从而获得一个端到端的自组装系统，导致拉曼信号放大一个数量级并大大提高了灵敏度。同时，生物传感器的外层涂有二氧化硅，这提高了系统的稳定性并便于其未来应用。当检测浓度在0.1 - 50 pg/mL范围内时，传感器产生的SERS信号与IL-5浓度呈现良好的线性关系。此外，它在稀释痰液和哮喘临床受试者中具有令人满意的性能，能够实现对气道炎症因子IL-5的灵敏检测。总体而言，所开发的基于SERS效应的生物传感器具有快速灵敏的检测性能优势，适用于监测痰液中的气道炎症因子。
