地榆和水飞蓟醇提物对的影响。

The Effect of Hydroalcoholic Extract of and Boiss Against .

机构信息

Ahvaz Jundishapur University of Medical Sciences, Department of Parasitology, Ahvaz, Iran.

Isfahan University Faculty of Medicine, Department of Parasitology, Isfahan, Iran.

出版信息

Turkiye Parazitol Derg. 2023 Dec 27;47(4):214-219. doi: 10.4274/tpd.galenos.2023.55264.

DOI:10.4274/tpd.galenos.2023.55264

PMID:38149441

Abstract

OBJECTIVE

Trichomoniasis is the most common sexually transmitted protozoan infection worldwide. Metronidazole is widely considered as the drug of choice for treating of trichomoniasis but considering its potential side effects, we aimed to assess the therapeutic influences of hydro-alcoholic extracts of and Boiss as alternative medications against .

METHODS

The trophozoites were cultured in TYI-S-33 medium at a density of 5x10 trophozoites/mL. Subsequently, they were incubated with varying concentrations of the plant extracts (32, 64, 125, 250, 500, and 1,000 μg/mL) and metronidazole (16, 32, 64, 125, 250, and 500 μg/mL), as the positive control. The number of trophozoites in each well plate was quantified after 2, 4, 6, 24, 48, and 72 hours using trypan blue staining. Finally, the viability of the parasite was assessed by vital methylene blue staining.

RESULTS

The hydro-alcoholic extracts of and Boiss at concentrations of 125, 250, 500, and 1,000 μg/mL demonstrated significant efficacy against the parasite. Our findings indicated that the minimum effective concentrations were 125 μg/mL and hydro-alcoholic extracts of and Boiss have the ability to effectively eliminate after 48 and 72 hours of treatment.

CONCLUSION

The findings of the present study showed that hydro-alcoholic extract of and Boiss can induce death in . However, further complementary in vivo studies are needed to assess the components of these plants in the treatment of .

摘要

目的

滴虫病是全球最常见的性传播原生动物感染。甲硝唑被广泛认为是治疗滴虫病的首选药物，但考虑到其潜在的副作用，我们旨在评估和 Boiss 的水醇提取物作为替代药物治疗的治疗影响。

方法

滋养体在 TYI-S-33 培养基中以 5x10 滋养体/mL 的密度培养。随后，将它们与不同浓度的植物提取物（32、64、125、250、500 和 1000μg/mL）和甲硝唑（16、32、64、125、250 和 500μg/mL）孵育，作为阳性对照。用台盼蓝染色在 2、4、6、24、48 和 72 小时后定量每个平板孔中的滋养体数量。最后，通过活甲基蓝染色评估寄生虫的活力。