Buzsáki G, Gage F H, Czopf J, Björklund A
Brain Res. 1987 Jan 6;400(2):334-47. doi: 10.1016/0006-8993(87)90632-9.
Solid pieces of the fetal septal region (SG) or hippocampus (HPC) were implanted in a cavity formed by aspiration of the fimbria fornix (FF) and the overlying cingulate cortex on one side in adult rats. In other lesioned animals cell suspensions obtained from the fetal septal area (SS) or the locus coeruleus region of the brainstem (LC) were injected stereotaxically into the deafferented host hippocampus. Six to 9 months after transplantation the animals had chronic recording electrodes implanted into both hippocampi. EEG and unit activity were monitored during running in a wheel, drinking and immobility. Unilateral fimbria-fornix lesions abolished rhythmic slow activity (RSA or theta, theta) in the ipsilateral hippocampus and no recovery was seen up to 9 months later in either the control FF-lesioned animals or in the rats with LC suspension grafts. Recovery of RSA, however, was observed in all animals with solid septal grafts and in some rats with solid HPC grafts. Similar to normal rats, RSA was present only during running and absent during drinking and sitting still. Coherence measurements of RSA between the transplanted and intact hemispheres resulted in high values (0.70-0.95). Concurrent with RSA, interneurons and granule cells in the host hippocampus fired rhythmically at RSA frequency (6-9 Hz). The depth profile and the antero-posterior distribution of the power of RSA correlated with the amount and distribution of the graft-induced acetylcholinesterase-positive reinnervation of the host hippocampus. In contrast to the animals with solid septal grafts, placed within the FF lesion cavity, the rats with intrahippocampal septal suspension grafts displayed only short duration bursts of RSA, and mainly during immobility. Based on these findings it is suggested that at least a proportion of the RSA 'pacemaker' cells of the host septum survives the transection of the fimbria-fornix fibers and that a graft of fetal septal or hippocampal tissue implanted into the lesion cavity may be capable of relaying this pacemaker activity to the host hippocampus. This effect may be due to the ability of the grafted tissue to promote the regeneration of new, direct or indirect, septo-hippocampal connections across the lesion cavity.
将胎儿隔区（SG）或海马体（HPC）的固体组织块植入成年大鼠一侧因抽吸穹窿伞（FF）及上方扣带回皮质而形成的腔隙中。在其他造模动物中，将从胎儿隔区（SS）或脑干蓝斑区域（LC）获取的细胞悬液立体定向注射到去传入神经支配的宿主海马体中。移植后6至9个月，给动物双侧海马体植入慢性记录电极。在动物转轮奔跑、饮水和静止不动期间监测脑电图（EEG）和单位活动。单侧穹窿伞损伤消除了同侧海马体中的节律性慢活动（RSA或θ波，θ），在对照的FF损伤动物或接受LC悬液移植的大鼠中，直至9个月后均未见恢复。然而，在所有接受固体隔区移植的动物以及部分接受固体HPC移植的大鼠中观察到了RSA的恢复。与正常大鼠相似，RSA仅在奔跑时出现，饮水和静止时消失。移植半球与完整半球之间RSA的相干测量结果显示数值较高（0.70 - 0.95）。与RSA同时出现的是，宿主海马体中的中间神经元和颗粒细胞以RSA频率（6 - 9赫兹）有节律地放电。RSA功率的深度分布和前后分布与移植诱导的宿主海马体乙酰胆碱酯酶阳性再支配的数量和分布相关。与置于FF损伤腔内的固体隔区移植动物不同，海马体内隔区悬液移植的大鼠仅表现出短时间的RSA爆发，且主要在静止不动时出现。基于这些发现，提示宿主隔区中至少一部分RSA“起搏器”细胞在穹窿伞纤维横断后存活下来，并且植入损伤腔内的胎儿隔区或海马体组织移植可能能够将这种起搏器活动传递给宿主海马体。这种效应可能归因于移植组织促进跨越损伤腔形成新的、直接或间接的隔 - 海马连接再生的能力。
