Chang Qing, Yang Yiwei, Hong Bo, Zhao Yanqun, Zhao Mengxin, Han Shanshan, Zhang Feng, Peng Huan, Peng Deliang, Li Yingmei
Shaanxi Key Laboratory of Plant Nematology, Bio-Agriculture Institute of Shaanxi, Xi'an, China.
Yulin Agricultural Technology Service Center, Yulin, China.
Front Plant Sci. 2023 Dec 13;14:1322902. doi: 10.3389/fpls.2023.1322902. eCollection 2023.
The potato rot nematode, , poses a serious threat to numerous root and tuber crops, yet the functional characterization of effectors from this migratory endoparasitic plant nematode remains limited. Despite inhabiting distinct habitats, sedentary and migratory plant parasitic nematodes share the structurally conserved effectors, such as venom allergen-like proteins (VAPs). In this study, a variant of was cloned from . The transcription profile analysis revealed that was higher expressed in feeding on either potato or sweet potato compared to on fungus via qRT-PCR. And was highly expressed at all life stages feeding on sweet potato, except for eggs. VAP2 was confirmed to be specifically expressed in the subventral esophageal glands of through hybridization assays. Combined with functional validation of the signal peptide of VAP2, it suggested that VAP2 could be secreted from nematode into host. Heterologous expression of VAP2 in revealed that the protein localized in both cytosol and nuclei of plant cells. Knocking down by RNAi in resulted in infection and reproduction defects on plants. All the results suggest that plays a crucial role in the interaction between and plants by facilitating the nematode infection.
马铃薯腐烂线虫对许多块根和块茎作物构成严重威胁，然而这种迁移性内寄生植物线虫效应子的功能特性研究仍然有限。尽管定居型和迁移型植物寄生线虫栖息于不同的生境，但它们共享结构保守的效应子，如类毒液过敏原蛋白（VAPs）。在本研究中，从[具体来源]克隆了一种[线虫种类]的变体。转录谱分析显示，通过qRT-PCR检测，与取食真菌相比，取食马铃薯或甘薯的[线虫种类]中[目标基因]表达量更高。并且在取食甘薯的所有生命阶段中，除了卵之外，[目标基因]均高表达。通过原位杂交试验证实VAP2在[线虫种类]的腹侧食管腺中特异性表达。结合VAP2信号肽的功能验证，表明VAP2可以从线虫分泌到宿主中。VAP2在[宿主植物种类]中的异源表达表明该蛋白定位于植物细胞的细胞质和细胞核中。通过RNAi在[线虫种类]中敲低[目标基因]导致其在植物上的侵染和繁殖出现缺陷。所有结果表明，[目标基因]通过促进线虫侵染在[线虫种类]与植物的相互作用中起关键作用。
