Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.
Penn State Cancer Institute, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, United States.
J Am Chem Soc. 2024 Jan 10;146(1):868-877. doi: 10.1021/jacs.3c11208. Epub 2023 Dec 28.
Great effort has been made to encapsulate or coat living mammalian cells for a variety of applications ranging from diabetes treatment to three-dimensional printing. However, no study has reported the synthesis of a biomimetic bacterial capsule to display high-affinity aptamers on the cell surface for enhanced cell recognition. Therefore, we synthesized an ultrathin alginate-polylysine coating to display aptamers on the surface of living cells with natural killer (NK) cells as a model. The results show that this coating-mediated aptamer display is more stable than direct cholesterol insertion into the lipid bilayer. The half-life of the aptamer on the cell surface can be increased from less than 1.5 to over 20 h. NK cells coated with the biomimetic bacterial capsule exhibit a high efficiency in recognizing and killing target cells. Therefore, this work has demonstrated a promising cell coating method for the display of aptamers for enhanced cell recognition.
人们已经付出了巨大的努力来封装或涂层活的哺乳动物细胞，以用于从糖尿病治疗到三维打印等各种应用。然而，目前还没有研究报道合成仿生细菌胶囊来在细胞表面展示高亲和力的适体，以增强细胞识别。因此，我们合成了一种超薄的藻酸盐-聚赖氨酸涂层，以自然杀伤 (NK) 细胞作为模型，在活细胞表面展示适体。结果表明，这种涂层介导的适体展示比直接将胆固醇插入脂质双层更稳定。细胞表面适体的半衰期可以从不到 1.5 小时增加到 20 小时以上。用仿生细菌胶囊包被的 NK 细胞在识别和杀伤靶细胞方面表现出很高的效率。因此，这项工作证明了一种有前途的细胞涂层方法，用于展示适体以增强细胞识别。
