Vandalem J L, Bodart C, Pirens G, Closset J, Hennen G
J Endocrinol. 1979 Apr;81(1):1-10. doi: 10.1677/joe.0.0810001.
Antisera were raised against highly purified preparations of porcine luteinizing hormone (pLH) and follicle-stimulating hormone (pFSH). Highly specific and sensitive radioimmunoassay systems were developed. The antisera to LH and FSH were used at working dilutions of 1:500,000 and 1:200,000 respectively and the sensitivities of the assays were 0.1 ng LH/ml serum (3 x 10(-12) mol/l) and 0.5 ng FSH/ml serum (1.5 x 10(-11) mol/l). The LH and FSH preparations used as standards were 1.2 and 81 times as potent as NIH-LH-S15 and NIH-FSH-P1 respectively. Both assays were validated and adapted for the measurement of the gonadotrophin content of porcine serum. The concentrations of LH and FSH in blood were measured simultaneously in prepubertal sows throughout a 24 h period, in adult sows during the oestrous cycle and in both prepubertal and adult animals after treatment with LH releasing hormone.
制备了针对高度纯化的猪促黄体生成素（pLH）和促卵泡激素（pFSH）制剂的抗血清。开发了高度特异且灵敏的放射免疫分析系统。用于LH和FSH的抗血清的工作稀释度分别为1:500,000和1:200,000，分析的灵敏度分别为0.1 ng LH/ml血清（3×10⁻¹² mol/l）和0.5 ng FSH/ml血清（1.5×10⁻¹¹ mol/l）。用作标准品的LH和FSH制剂的效价分别是NIH-LH-S15和NIH-FSH-P1的1.2倍和81倍。两种分析方法均经过验证，并适用于测定猪血清中的促性腺激素含量。在整个24小时期间，对青春期前母猪、发情周期中的成年母猪以及用促黄体生成素释放激素处理后的青春期前和成年动物同时测定血液中LH和FSH的浓度。
