Tassava R A, Garling D J
J Exp Zool. 1979 Apr;208(1):97-110. doi: 10.1002/jez.1402080111.
Complete flaps of whole skin over the amputation surface of larval axolotl limbs did not prevent dedifferentiation, DNA synthesis, and mitosis. However, even by two weeks, insufficient cell division had occurred for blastema formation. In about 50% of the cases, small areas of wound epidermis formed over the amputated tip of the radius or ulna. At two weeks after amputation, mitotic figures and dedifferentiated cells which incorporated H3-thymidine were predominantly located in the vicinity of the small area of wound epidermis. Although delayed in relation to controls, these limbs regenerated. The results are consistent with the view that the wound epidermis is necessary to maintain dedifferentiated cells in the cell cycle.
在幼体美西螈肢体截肢表面覆盖完整的全层皮肤瓣并不能阻止细胞去分化、DNA合成和有丝分裂。然而，即使到两周时，形成芽基的细胞分裂仍不足。在大约50%的病例中，桡骨或尺骨截肢端上方形成了小面积的伤口表皮。截肢后两周，有丝分裂图像和掺入H3-胸腺嘧啶核苷的去分化细胞主要位于伤口表皮小面积区域附近。尽管相对于对照组有所延迟，但这些肢体仍实现了再生。这些结果与伤口表皮对于维持去分化细胞处于细胞周期中是必需的这一观点一致。
