Dai Yi, Yang Fanqin, Li Jie, Fu Huinan, Wang Xi, Wan Bin, Cai Meiting, Xin Tianrong, Xia Bin, Zhong Ling, Zou Zhiwen
School of Life Science, Nanchang University, Nanchang 330031, China.
Industry Development & Service Center for Agriculture and Rural Affairs of Jiangxi Province, Nanchang 330096, China.
J Agric Food Chem. 2024 Jan 10;72(1):200-208. doi: 10.1021/acs.jafc.3c06892. Epub 2023 Dec 30.
, one of the most destructive corn pests in the world, invaded China in December 2018. In this study, sublethal concentrations (LC and LC) of emamectin benzoate (EB) were used to treat pesticide-free treatment (PFT) and EB treatment (ET) of . In PFT, compared with the control (CK), the pupal weight, hatching rate, and pupation rate of LC and LC groups were significantly reduced. The fecundity and the expression of vitellogenin gene () were decreased after LC treatment, while the LC treatment groups showed no significant difference from the control group. In ET, compared to CK, the fecundity was increased by 11.14 and 18.8%. The expression of was upregulated by 2.6 times after LC treatment. Moreover, RNAi-mediated knockdown resulted in a nearly 70% reduction in oviposition. The result provided a theoretical basis for optimizing the application of EB and Vg-dsRNA in the control of .
[某种害虫名称]是世界上最具破坏性的玉米害虫之一,于2018年12月侵入中国。在本研究中,使用甲氨基阿维菌素苯甲酸盐(EB)的亚致死浓度(LC和LC)对[害虫名称]进行无农药处理(PFT)和EB处理(ET)。在PFT中,与对照(CK)相比,LC和LC组的蛹重、孵化率和化蛹率显著降低。LC处理后,产卵量和卵黄原蛋白基因()的表达降低,而LC处理组与对照组无显著差异。在ET中,与CK相比,产卵量分别增加了11.14%和18.8%。LC处理后,的表达上调了2.6倍。此外,RNAi介导的基因敲除导致产卵量降低了近70%。该结果为优化EB和Vg-dsRNA在[害虫名称]防治中的应用提供了理论依据。