Renu Swathi Manohar, Rao Ashwin P, Biranthabail Dhanashree, Natarajan Srikant, Mahabala Karuna Yarmuja, Panakaje Anupama
Department of Pediatric and Preventive Dentistry, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.
Department of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.
Int J Clin Pediatr Dent. 2023 Sep-Oct;16(5):678-680. doi: 10.5005/jp-journals-10005-2614.
Multidose vials (MDVs) for local anesthetic injections are routinely used in dental practice. MDVs contain multiple doses of a parenteral drug intended for administration to the same or multiple patients. Potentially harmful microbes have been shown to be able to live and occasionally multiply in MDVs if not handled aseptically. The goal of this study thus was to evaluate the bacterial and fungal contamination (FC) of lignocaine MDVs after use in a dental hospital.
A total of 27 MDVs of lignocaine free of any microbial contamination were distributed to different departments of the dental hospital and they were asked to use them on patients routinely. The samples were recollected from the departments either at the end of the 28th day or as and when the contents in the MDVs reached a predetermined level marked on the bottle during its usage, whichever was earlier. These leftover samples were subjected to a microbiological investigation by inoculation into thioglycolate broth and subsequent subculturing onto agar plates.
None of the inoculated broths showed turbidity. Subcultured agar plates, even on the 7th day of incubation, did not show any bacterial or fungal growth. The lignocaine MDVs tested after use were thus found to be sterile.
There was no bacterial or FC detected in MDVs of lignocaine subjected to testing after use in the dental hospital.
Multidose vials MDVs) continue to be used in clinics for economic reasons. But the clinician opting for MDVs should be conscious of their potential for microbial contamination and should meticulously follow the protocol for their aseptic use.
Renu SM, Rao AP, Biranthabail D, Screening for Microbial Contamination of Multi-dose Lignocaine Vials in a Dental Hospital: A Prospective Study. Int J Clin Pediatr Dent 2023;16(5):678-680.
用于局部麻醉注射的多剂量瓶（MDV）在牙科实践中经常使用。MDV含有多剂量的胃肠外给药药物，供同一患者或多名患者使用。如果不进行无菌操作，已证明潜在有害微生物能够在MDV中存活并偶尔繁殖。因此，本研究的目的是评估利多卡因MDV在牙科医院使用后的细菌和真菌污染（FC）情况。
将27个无任何微生物污染的利多卡因MDV分发给牙科医院的不同科室，并要求他们常规用于患者。样本在第28天结束时或在MDV中的内容物在使用期间达到瓶子上标记的预定水平时（以较早者为准）从各科室重新收集。将这些剩余样本接种到硫乙醇酸盐肉汤中进行微生物学调查，随后再接种到琼脂平板上进行传代培养。
接种的肉汤均未出现浑浊。即使在培养的第7天，传代培养的琼脂平板上也未显示任何细菌或真菌生长。因此，使用后测试的利多卡因MDV被发现是无菌的。
在牙科医院使用后进行测试的利多卡因MDV中未检测到细菌或FC。
出于经济原因，多剂量瓶（MDV）仍在临床中使用。但选择使用MDV的临床医生应意识到其微生物污染的可能性，并应严格遵循其无菌使用方案。
Renu SM, Rao AP, Biranthabail D, 牙科医院多剂量利多卡因瓶微生物污染的筛查：一项前瞻性研究。《国际临床儿科牙科学杂志》2023；16(5):678 - 680。
