Ammon H P, Verspohl E J
Diabetologia. 1979 Jul;17(1):41-4. doi: 10.1007/BF01222976.
Methylene Blue, which is known to oxidise NADPH in red blood cells, was used to assess a possible role of NADPH in the glucose-stimulated secretion of insulin. When islets from rats were incubated with 3 mg/ml of glucose, Methylene Blue (0.5), 1.0, 2.0 or 5.0 micrograms/ml) significantly decreased the concentration of NADPH, increased that of NADP+ and decreased the NADPH/NADP+ ratio in a dose-dependent manner. This effect was associated with inhibition of the glucose-induced insulin release. No significant change of NADH, NAD+ and ATP could be observed. It is suggested that the secretory response of the pancreatic islet to glucose stimulation depends on the NADPH/NADP+ ratio.
已知亚甲蓝可氧化红细胞中的NADPH，因此被用于评估NADPH在葡萄糖刺激胰岛素分泌过程中可能发挥的作用。当将大鼠胰岛与3mg/ml葡萄糖、0.5、1.0、2.0或5.0μg/ml亚甲蓝共同孵育时，亚甲蓝会以剂量依赖的方式显著降低NADPH浓度，升高NADP⁺浓度，并降低NADPH/NADP⁺比值。这种效应与葡萄糖诱导的胰岛素释放受到抑制有关。未观察到NADH、NAD⁺和ATP有显著变化。提示胰岛对葡萄糖刺激的分泌反应取决于NADPH/NADP⁺比值。
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