Ammon H P, Akhtar M S, Grimm A, Niklas H
Naunyn Schmiedebergs Arch Pharmacol. 1979 May;307(1):91-6. doi: 10.1007/BF00506556.
Methylene blue (an oxidant of NADPH), diamide (an oxidant of glutathion-SH [GSH]) and tertbutyl hydroperoxide (a substrate of glutathione peroxidase) significantly decreased the GSH content of pancreatic rat islets and decreased their GSH/GSSG ratio. They also significantly depressed the single peak insulin response to tolbutamide by the isolated perfused pancreas as well as its synergistic action with glucose in isolated pancreatic islets. These results suggest that the effect of tolbutamide alone and its synergistic action with glucose could depend on the islet NADPH and GSH. In addition it appears that augmentation of tolbutamide action by glucose in insulin release is mediated by the provision of additional NADPH and GSH through glucose metabolism.
亚甲蓝（一种烟酰胺腺嘌呤二核苷酸磷酸[NADPH]的氧化剂）、二酰胺（一种谷胱甘肽-SH[GSH]的氧化剂）和叔丁基过氧化氢（一种谷胱甘肽过氧化物酶的底物）显著降低了大鼠胰腺胰岛的谷胱甘肽含量，并降低了它们的谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值。它们还显著抑制了离体灌注胰腺对甲苯磺丁脲的单峰胰岛素反应及其在离体胰岛中与葡萄糖的协同作用。这些结果表明，甲苯磺丁脲单独的作用及其与葡萄糖的协同作用可能取决于胰岛中的NADPH和谷胱甘肽。此外，似乎葡萄糖在胰岛素释放中增强甲苯磺丁脲作用是通过葡萄糖代谢提供额外的NADPH和谷胱甘肽来介导的。
