Akbari Abolfazl, Jelodar Gholamali, Hosseinzadeh Saeid
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Basic Med Sci. 2024;27(1):39-48. doi: 10.22038/IJBMS.2023.69324.15110.
High levels of resistin are associated with metabolic diseases and their complications, including hypertension. The paraventricular nucleus (PVN) is also involved in metabolic disorders and cardiovascular diseases, such as hypertension. Therefore, this study aimed to study cardiovascular (CV) responses evoked by the injection of resistin into the lateral ventricle (LV) and PVN and determine the mechanism of these responses in the rostral ventrolateral medulla (RVLM).
Arterial pressure (AP) and heart rate (HR) were evaluated in urethane-anesthetized male rats (1.4 g/kg intraperitoneally) before and after all injections. This study was carried out in two stages. Resistin was injected into LV at the first stage, and AP and HR were evaluated. After that, the paraventricular, supraoptic, and dorsomedial nuclei of the hypothalamus were chosen to evaluate the gene expression of c-Fos. Afterward, resistin was injected into PVN, and cardiovascular responses were monitored. Then to detect possible neural mechanisms of resistin action, agonists or antagonists of glutamatergic, GABAergic, cholinergic, and aminergic transmissions were injected into RVLM.
Resistin injection into LV or PVN could increase AP and HR compared to the control group and before injection. Resistin injection into LV also increases the activity of RVLM, paraventricular, supraoptic, and dorsomedial areas. Moreover, the CV reflex created by the administration of resistin in PVN is probably mediated by glutamatergic transmission within RVLM.
It can be concluded that hypothalamic nuclei, including paraventricular, are important central areas for resistin actions, and glutamatergic transmission in RVLM may be one of the therapeutic targets for high AP in obese people or with metabolic syndrome.
高水平的抵抗素与代谢性疾病及其并发症（包括高血压）相关。室旁核（PVN）也参与代谢紊乱和心血管疾病，如高血压。因此，本研究旨在研究向侧脑室（LV）和室旁核注射抵抗素所诱发的心血管（CV）反应，并确定这些反应在延髓头端腹外侧区（RVLM）的机制。
在所有注射前后，对经乌拉坦麻醉的雄性大鼠（腹腔注射1.4 g/kg）的动脉压（AP）和心率（HR）进行评估。本研究分两个阶段进行。第一阶段向侧脑室注射抵抗素，并评估动脉压和心率。之后，选择下丘脑的室旁核、视上核和背内侧核来评估c-Fos的基因表达。随后，向室旁核注射抵抗素，并监测心血管反应。然后，为检测抵抗素作用可能的神经机制，将谷氨酸能、γ-氨基丁酸能、胆碱能和胺能传递的激动剂或拮抗剂注射到延髓头端腹外侧区。
与对照组和注射前相比，向侧脑室或室旁核注射抵抗素可增加动脉压和心率。向侧脑室注射抵抗素还可增加延髓头端腹外侧区、室旁核、视上核和背内侧区的活性。此外，在室旁核注射抵抗素所产生的心血管反射可能由延髓头端腹外侧区内的谷氨酸能传递介导。
可以得出结论，包括室旁核在内的下丘脑核是抵抗素作用的重要中枢区域，延髓头端腹外侧区的谷氨酸能传递可能是肥胖或患有代谢综合征的高血压患者的治疗靶点之一。
