Rivas L A, Pont Lezica R
Eur J Biochem. 1987 Feb 16;163(1):129-34. doi: 10.1111/j.1432-1033.1987.tb10745.x.
Membrane preparations from the non-photosynthetic alga Prototheca zopfii incorporate glucose from UDP-[3H]glucose into the trichloroacetic-acid-insoluble fraction and the polysaccharides insoluble in hot alkali. Time course and pulse-chase experiments indicate that the acid-insoluble fraction was a precursor of the alkali-insoluble fraction. Isolation of 3H-labeled membrane or soluble fraction showed that only membrane fractions were able to transfer radioactivity into polysaccharides. Treatment of glucosylated membranes with trypsin or cellulase only partially affect their transfer ability, indicating that the precursor was internalized in vesicles. Analysis of the in vitro synthesized polysaccharides by enzymatic and acid hydrolysis showed that glucose and cellobiose were present as radioactive sugars. Permethylation of the polysaccharide indicates that 80% of the glucose was beta-1,4-bonded with 20% in beta-1,3-linkages. This polysaccharide was found to be identical with the cell-wall beta-glucan obtained in vivo [Rivas, L.A. & Pont Lezica, R. (1978) Planta (Berl.) 165, 348-353].
从非光合藻类原壁菌（Prototheca zopfii）制备的膜制剂能将UDP-[3H]葡萄糖中的葡萄糖掺入三氯乙酸不溶性部分和热碱不溶性多糖中。时间进程和脉冲追踪实验表明，酸不溶性部分是碱不溶性部分的前体。对3H标记的膜或可溶性部分的分离表明，只有膜部分能够将放射性转移到多糖中。用胰蛋白酶或纤维素酶处理糖基化膜只会部分影响其转移能力，这表明前体被内化到囊泡中。通过酶解和酸水解对体外合成的多糖进行分析表明，葡萄糖和纤维二糖作为放射性糖存在。多糖的全甲基化表明，80%的葡萄糖以β-1,4键连接，20%以β-1,3键连接。发现这种多糖与体内获得的细胞壁β-葡聚糖相同[里瓦斯，L.A. & 庞特·莱齐卡，R.（1978年）《植物（柏林）》165，348 - 353]。
