Vater J, Schlumbohm W, Palacz Z, Salnikow J, Gadow A, Kleinkauf H
Eur J Biochem. 1987 Mar 2;163(2):297-302. doi: 10.1111/j.1432-1033.1987.tb10800.x.
The preparation of both enzymes of gramicidin S synthetase was efficiently improved by introduction of the fast protein liquid chromatography technique. High-resolution anion-exchange chromatography on Pharmacia Mono Q HR 5/5 was used as the final purification step. D-Phe-Pro-Val-cyclo-Orn was obtained as a product of the multienzyme by omission of L-leucine from the complete bioassay mixture. This tetrapeptide was formed by cyclization of the C-terminal ornithine to 3-amino-2-piperidone. It was identified and characterized by chromatographic and spectroscopic procedures using chemically synthesized reference compounds.
