良性和恶性前列腺肿瘤表现出不同的胶原空间组织。

Benign and malignant prostate neoplasms show different spatial organization of collagen.

机构信息

Taras Zadvornyi, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasylkivska str. 45, Kyiv-03022, Ukraine,

出版信息

Croat Med J. 2023 Dec 31;64(6):413-420. doi: 10.3325/cmj.2023.64.413.

DOI:10.3325/cmj.2023.64.413

PMID:38168522

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10797232/

Abstract

AIM

To compare the indicators of the spatial organization of collagen and its regulating factors between benign and malignant prostate neoplasms.

METHODS

The study involved tumor tissue samples from 40 patients with stage II-III prostate cancer (PCa) and 20 patients with benign prostatic hyperplasia (BPH). The localization of collagen was determined with a Masson trichrome stain. To establish quantitative indicators of the spatial organization of collagen, morphometric studies were carried out with the CurveAlign and ImageJ programs.

RESULTS

PCa tissue had two times lower collagen density (P<0.0001) and 1.3 times lower levels of collagen alignment (P=0.018) compared with BPH tissue. In PCa tissue, collagen fibers were shorter (by 24.2%; P<0.001) and thicker (by 15.5%; P<0.001). PCa tissue samples showed significantly higher levels of metalloproteinase (MMP)-2 (by 2.4 times; P=0.001), MMP-8 (by 2.3 times; P=0.007), and MMP-13 (by 1.9 times; P=0.004).

CONCLUSIONS

Collagen matrix spatial organization features, as well as its regulatory factors, could be potential biomarkers of malignant prostate neoplasms.

摘要

目的

比较良性和恶性前列腺肿瘤组织中胶原蛋白及其调节因子的空间组织学指标。

方法

本研究纳入了 40 名 II-III 期前列腺癌（PCa）患者和 20 名良性前列腺增生（BPH）患者的肿瘤组织样本。采用 Masson 三色染色法确定胶原蛋白的定位。为了建立胶原蛋白空间组织学的定量指标，采用 CurveAlign 和 ImageJ 程序进行形态计量学研究。

结果

与 BPH 组织相比，PCa 组织的胶原蛋白密度低 2 倍（P<0.0001），胶原蛋白排列水平低 1.3 倍（P=0.018）。在 PCa 组织中，胶原蛋白纤维短 24.2%（P<0.001）且厚 15.5%（P<0.001）。PCa 组织样本中金属基质蛋白酶（MMP）-2 的水平高 2.4 倍（P=0.001），MMP-8 高 2.3 倍（P=0.007），MMP-13 高 1.9 倍（P=0.004）。