Yamoto M, Nakano R
Exp Clin Endocrinol. 1986 Nov;88(1):45-9. doi: 10.1055/s-0029-1210573.
The binding of 125I-labelled PRL to the porcine granulosa cells was examined. Displacement studies demonstrated that 125I-labelled PRL was specifically bound to the granulosa cells. The specific binding of 125I-labelled PRL to the granulosa cells decreased significantly as the follicles matured. The addition of diethylstilboestrol diphosphate enhanced the binding of 125I-labelled PRL to the granulosa cells harvested from small, medium and large follicles. The results of the present study suggest that oestrogen might have an important role in modulating PRL receptors in porcine granulosa cells.
研究了125I标记的催乳素（PRL）与猪颗粒细胞的结合情况。置换研究表明，125I标记的PRL能特异性地结合到颗粒细胞上。随着卵泡成熟，125I标记的PRL与颗粒细胞的特异性结合显著降低。添加己烯雌酚二磷酸可增强125I标记的PRL与从小、中、大卵泡采集的颗粒细胞的结合。本研究结果提示，雌激素可能在调节猪颗粒细胞中的PRL受体方面发挥重要作用。
