用环己酰亚胺处理的G1期早期、中期或晚期WI38细胞对HeLa细胞DNA合成诱导剂反应的差异。

Differences in the response of early and mid or late G1 WI38 cells treated with cyclohexamide to HeLa inducers of DNA synthesis.

作者信息

Rao M V

出版信息

Exp Cell Res. 1987 Mar;169(1):105-10. doi: 10.1016/0014-4827(87)90229-1.

Abstract

The inducibility of DNA synthesis after treatment with cyclohexamide (CHM) during mitosis and the G1 phase of WI38 cells has been studied in the heterokaryons following fusion with HeLa cells in S phase. Synchronized mitotic cells treated for up to 5 h with CHM were not delayed in the initiation of DNA synthesis in the heterokaryons. The G1 cells treated with CHM for 3-24 h were slow in responding to inducers of DNA synthesis generated by HeLa cells in the heterokaryons. The results suggest that there is a specific point in early G1 that regulates the entry of cells into a cycling state. In the presence of CHM, mitotic cells divide, but the daughter cells fail to enter G1 leading to DNA synthesis, and CHM treatment of G1 cells results in their transient entry into a G0 state.

摘要

在WI38细胞有丝分裂期和G1期用环己酰亚胺（CHM）处理后DNA合成的诱导性，已在与处于S期的HeLa细胞融合后的异核体中进行了研究。用CHM处理长达5小时的同步化有丝分裂细胞在异核体中DNA合成起始时没有延迟。用CHM处理3 - 24小时的G1期细胞对异核体中HeLa细胞产生的DNA合成诱导物反应迟缓。结果表明，在G1早期存在一个特定点，它调节细胞进入循环状态。在CHM存在的情况下，有丝分裂细胞分裂，但子细胞未能进入导致DNA合成的G1期，并且用CHM处理G1期细胞会导致它们短暂进入G0状态。