大鼠肝脏再生过程中瞬时诱导的mRNA序列的分子克隆
Molecular cloning of mRNA sequences transiently induced during rat liver regeneration.
作者信息
Sobczak J, Lotti A M, Taroux P, Duguet M
出版信息
Exp Cell Res. 1987 Mar;169(1):47-56. doi: 10.1016/0014-4827(87)90223-0.
In order to isolate genes which are induced during liver regeneration, we have constructed a cDNA library from 16-h-regenerating liver poly(A)+ RNA. By computer analysis of autoradiograms produced by differential plaque hybridization with cDNA from normal or 16-h-regenerating liver, we have isolated several recombinant clones representing sequences transiently increased during liver regeneration. Three of these were further characterized: the level of the corresponding mRNAs increase rapidly after partial hepatectomy, before the onset of DNA synthesis. Two clones were identified as fibrinogen clones. It is speculated that alpha-fibrinogen may be involved in the growth process, or in its regulation.
为了分离在肝脏再生过程中被诱导的基因，我们用16小时再生肝的多聚腺苷酸加尾RNA构建了一个cDNA文库。通过对用正常肝脏或16小时再生肝脏的cDNA进行差异噬菌斑杂交产生的放射自显影片进行计算机分析，我们分离出了几个代表肝脏再生过程中瞬时增加序列的重组克隆。其中三个被进一步鉴定：相应mRNA的水平在部分肝切除后、DNA合成开始前迅速升高。两个克隆被鉴定为纤维蛋白原克隆。据推测，α-纤维蛋白原可能参与生长过程或其调节。
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