Aquatic Environmental Biotechnology and Nanotechnology Division, ICAR-Central Inland, Fisheries Research Institute, Kolkata, West Bengal, India.
Department of Instrumentation and Electronics Engineering, Jadavpur University Salt Lake Campus, Kolkata, India.
PLoS One. 2024 Jan 3;19(1):e0295687. doi: 10.1371/journal.pone.0295687. eCollection 2024.
Due to the increase in urbanization and industrialization, the load of toxicants in the environment is alarming. The most common toxicants, including heavy metals and metalloids such as hexavalent Chromium, have severe pathophysiological impacts on humans and other aquatic biotas. Therefore, developing a portable rapid detection device for such toxicants in the aquatic environment is necessary. This work portrays the development of a field-portable image analysis device coupled with 3,3',5,5'-tetramethylbenzidine (TMB) as a sensing probe for chromium (VI) detection in the aquatic ecosystem. Sensor parameters, such as reagent concentration, reaction time, etc., were optimized for the sensor development and validation using a commercial UV-Vis spectrophotometer. The chemoreceptor integrated with a uniform illumination imaging system (UIIS) revealed the system's applicability toward Cr(VI) detection. The calibration curve using the R-value of image parameters allows Cr(VI) detection in the linear range of 25 to 600 ppb, which covers the prescribed permissible limit by various regulatory authorities. Furthermore, the adjusted R2 = 0.992 of the linear fit and correlation coefficients of 0.99018 against the spectrophotometric method signifies the suitability of the developed system. This TMB-coupled field-portable sensing system is the first-ever reported image analysis-based technology for detecting a wide range of Cr(VI) in aquatic ecosystems to our knowledge.
由于城市化和工业化的增加，环境中的有毒物质负荷令人震惊。最常见的有毒物质，包括重金属和类金属如六价铬，对人类和其他水生生物群具有严重的病理生理影响。因此，开发一种用于检测水生环境中此类有毒物质的便携式快速检测装置是必要的。本工作描述了一种现场便携式图像分析装置的开发，该装置与 3,3',5,5'-四甲基联苯胺 (TMB) 作为传感探针结合使用，用于检测水生生态系统中的六价铬 (Cr(VI))。使用商业紫外可见分光光度计对传感器进行了优化，包括试剂浓度、反应时间等传感器参数。将化学感受器与均匀照明成像系统 (UIIS) 集成，显示了该系统在 Cr(VI)检测方面的适用性。使用图像参数的 R 值绘制校准曲线，可以在 25 至 600 ppb 的线性范围内检测 Cr(VI)，该范围涵盖了各种监管机构规定的允许限值。此外，线性拟合的调整 R2 = 0.992 和与分光光度法的相关系数为 0.99018，表明开发的系统是合适的。据我们所知，这种与 TMB 结合的现场便携式传感系统是首次报道的用于检测水生生态系统中广泛范围的 Cr(VI)的基于图像分析的技术。
