Champier J, Claustrat B, Sassolas G, Berger M
FEBS Lett. 1987 Feb 23;212(2):220-4. doi: 10.1016/0014-5793(87)81348-0.
Immunoperoxidase electrophoresis was applied to the plasma of a patient showing a high level of prolactin (PRL) secreted by a pituitary adenoma. Two PRL monomers were detected with an anti-hPRL antiserum: a major 22 kDa form and a minor 25 kDa form. Concanavalin A-Sepharose 4B chromatography revealed that the 25 kDa form was a glycosylated variant of PRL. Incubation of this variant with endoglycosidase F led to its transformation into the 22 kDa form.
免疫过氧化物酶电泳应用于一名垂体腺瘤分泌高水平催乳素（PRL）患者的血浆。用抗人PRL抗血清检测到两种PRL单体：主要的22 kDa形式和次要的25 kDa形式。伴刀豆球蛋白A-琼脂糖4B层析显示，25 kDa形式是PRL的糖基化变体。该变体与内切糖苷酶F孵育后转化为22 kDa形式。
