The proteins in Lumbricus terrestris and Eisenia foetida coelomic fluids and on coelomocytes reacting with sheep and goat IgG molecules.

Sheep and goat fast migrating IgG molecules and their F(ab')2 fragments react with L. terrestris and E. foetida coelomic fluid proteins. The reaction was not mediated by antibody binding site but by some other part of the IgG molecule, since affinity purified antibodies of different antigen specificities as well as IgG preparations from normal sheep sera reacted with the same coelomic fluid proteins. The presence of at least two reacting proteins was detected by immunoelectrophoresis and immunoblotting analysis. Molecular weights of these proteins were 47 and 50 kD in L. terrestris and 42 kD in E. foetida. Sheep antibodies and IgG molecules also bind to the Sheep antibodies and IgG molecules also bind to the surface of about 2% of coelomic leukocytes and the immunoblotting analysis showed that the IgG binding proteins in coelomic fluids and in cell lysates were similar.