赤子爱胜蚓和陆正蚓体腔液中的溶解活性。

Lytic activities in coelomic fluid of Eisenia foetida and Lumbricus terrestris.

作者信息

Tucková L, Rejnek J, Síma P, Ondrejová R

出版信息

Dev Comp Immunol. 1986 Spring;10(2):181-9. doi: 10.1016/0145-305x(86)90002-9.

Abstract

Coelomic fluids of the two earthworm species E.foetida (E.F.) and L.terrestris (L.T.) have not only the ability to lyse various vertebrate erythrocytes but also to digest vertebrate serum proteins. Both activities are carried by different molecules since hemolysis but not proteolysis was inhibited by simple sugars. In contrary, proteolysis was blocked by PMSF which did not influence hemolysis. Coelomic fluids of E.F. digest effectively vertebrate serum proteins (PIgG, HSA) but not the proteins of L.T. coelomic fluids. The proteolytic activity was detected in approximately 40 000 mol. wt. fraction. After digestion proteolytic fragments were analyzed by immunoelectrophoresis, SDS-PAGE and TCA precipitation. Two of the fragments reacting with PIgG antisera remained intact even after 120 h digestion.

摘要

两种蚯蚓，即赤子爱胜蚓（E.F.）和陆正蚓（L.T.）的体腔液不仅具有溶解多种脊椎动物红细胞的能力，还能消化脊椎动物血清蛋白。这两种活性由不同分子承担，因为简单糖类可抑制溶血但不抑制蛋白水解。相反，苯甲基磺酰氟（PMSF）可阻断蛋白水解，而不影响溶血。E.F.的体腔液能有效消化脊椎动物血清蛋白（猪免疫球蛋白G、人血清白蛋白），但不能消化L.T.体腔液中的蛋白质。在约40000分子量的组分中检测到蛋白水解活性。消化后，通过免疫电泳、十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和三氯乙酸沉淀分析蛋白水解片段。即使在消化120小时后，与猪免疫球蛋白G抗血清反应的两个片段仍保持完整。