Shahsavari Mohammad Hamed, Moghaddam Gholamali, Alves Kele Amaral, Alves Benner Geraldo, de Lima Laritza Ferreira, Azimi Golshan, Berrocal Deysi Juana Dipaz, Silva Luciana Mascena, da Silva Yago Pinto, Vizcarra Diego Alberto Montano, de Figueiredo José Ricardo, Rodrigues Ana Paula Ribeiro
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Gynecology and Obstetrics, Medicine College, University of São Paulo, Ribeirão Preto, Brazil.
Vet Res Forum. 2023;14(12):673-679. doi: 10.30466/vrf.2023.1972637.3684. Epub 2023 Dec 15.
Although cryopreservation of ovarian tissue has advanced greatly, it remains a challenge, and protocols should be optimized to handle the heterogeneous nature of ovarian samples. In an effort to address this factor, the present study evaluated the effects of corpus luteum (CL) and side of ovaries (right versus left) on cellular morphology and viability of vitrified bovine ovarian fragments in a closed system. The ovaries were categorized according to whether they had a CL and which side they were on, and then divided into six groups: 1) CL+ (with CL) group; 2) CL- (without CL) group; 3) right ovaries group; 4) left ovaries group; 5) fresh control group (ovaries without vitrification or culture that were not selected for CL or ovarian side) and 6) culture medium control group (non-vitrified ovaries that were not selected for the presence or absence of CL or side of the ovaries). The current study shows that the CL- and right groups had the greatest percentage of follicles with normal morphology compared to other vitrified-warmed groups. Furthermore, the levels of necrosis and tissue damage of the right cultured group were the lowest compared to other groups. It was shown that bovine ovarian tissues derived from right ovaries and ovaries without a corpus luteum can be functionally and morphologically preserved after vitrification. For the first time, the present study suggests that bovine ovarian tissue vitrification can be improved by considering the origin of the ovaries.
尽管卵巢组织的冷冻保存技术已经取得了很大进展，但它仍然是一个挑战，并且应该优化方案以处理卵巢样本的异质性。为了解决这个因素，本研究在一个封闭系统中评估了黄体（CL）和卵巢侧别（右侧与左侧）对玻璃化牛卵巢碎片细胞形态和活力的影响。根据卵巢是否有黄体以及所在侧别进行分类，然后分为六组：1）CL+（有黄体）组；2）CL-（无黄体）组；3）右侧卵巢组；4）左侧卵巢组；5）新鲜对照组（未进行玻璃化或培养、未根据黄体或卵巢侧别选择的卵巢）和6）培养基对照组（未根据黄体存在与否或卵巢侧别选择的未玻璃化卵巢）。当前研究表明，与其他玻璃化-复温组相比，CL-组和右侧组形态正常的卵泡百分比最高。此外，与其他组相比，右侧培养组的坏死和组织损伤水平最低。结果表明，来自右侧卵巢和无黄体的牛卵巢组织在玻璃化后在功能和形态上可以得到保存。本研究首次表明，考虑卵巢的来源可以改善牛卵巢组织的玻璃化。
