异源促性腺激素（hCG）刺激的欧洲鳗鲡（Anguilla anguilla L.）的睾丸细胞学结构、血浆性类固醇和性腺细胞质游离类固醇受体

Testis cytological structure, plasma sex steroids, and gonad cytosol free steroid receptors of heterologous gonadotropin (hCG)-stimulated silver eel, Anguilla anguilla L.

作者信息

Colombo G, Grandi G, Romeo A, Giovannini G, Pelizzola D, Catozzi L, Piffanelli A

出版信息

Gen Comp Endocrinol. 1987 Feb;65(2):167-78. doi: 10.1016/0016-6480(87)90162-6.

Abstract

Complete testicular maturation was induced in silver eels, kept at 24 degrees in fresh water, by a single injection of 1000 I.U. of heterologous gonadotropin (hCG). Each week, for 4 weeks, some eels were examined for testis structural pattern, plasma sex steroids, and gonad cytosol steroid receptors. The first effect of the hCG was on the tubular organization of the testis, followed by spermatogenesis. Plasma androgens were not detectable in the untreated eels, whereas a peak was detected a week after in those treated with the injection and afterward a decline. Plasma progesterone and estradiol showed a peak 2 weeks after treatment. Untreated eel gonads showed a high content of cytosolic free estradiol receptors which disappeared in the hCG-treated ones, a peak of free progesterone receptors was found 1 week after injection. The results are discussed in relation to the differentiation and maturation of eels testes.

摘要

通过单次注射1000国际单位的异源促性腺激素（hCG），可诱导饲养在24摄氏度淡水中的银鳗实现睾丸完全成熟。在4周的时间里，每周都会对部分鳗鱼进行检查，以观察睾丸结构模式、血浆性类固醇以及性腺胞质溶胶类固醇受体的情况。hCG的首要作用是影响睾丸的管状组织，随后是精子发生。在未处理的鳗鱼中检测不到血浆雄激素，而在注射处理后的鳗鱼中，一周后检测到一个峰值，随后下降。血浆孕酮和雌二醇在处理后两周出现峰值。未处理的鳗鱼性腺显示出高含量的胞质游离雌二醇受体，而在hCG处理的鳗鱼中这些受体消失，注射后1周发现游离孕酮受体出现峰值。结合鳗鱼睾丸的分化和成熟对结果进行了讨论。