大麻素受体 2 在正畸牙齿移动过程中的骨重塑中的作用。
The role of cannabinoid receptor 2 in bone remodeling during orthodontic tooth movement.
机构信息
Department of Orthodontics, School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology & Hebei Clinical Research Center for Oral Diseases, East 383 Zhongshan Road, Shijiazhuang, Hebei Province, 050017, China.
The Key Laboratory of Stomatology, School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, Clinical Research Center for Oral Diseases, East 383 Zhongshan Road, Shijiazhuang, Hebei Province, 050017, China.
出版信息
BMC Oral Health. 2024 Jan 4;24(1):23. doi: 10.1186/s12903-023-03810-5.
BACKGROUND
The purpose of this study is to explore the effects of CB2 on bone regulation during orthodontic tooth movement.
METHODS
Thirty male mice were allocated into 2 groups (n = 15 in each group): wild type (WT) group and CB2 knockout (CB2) group. Orthodontic tooth movement (OTM) was induced by applying a nickel-titanium coil spring between the maxillary first molar and the central incisors. There are three subgroups within the WT groups (0, 7 and 14 days) and the CB2 groups (0, 7 and 14 days). 0-day groups without force application. Tooth displacement, alveolar bone mass and alveolar bone volume were assessed by micro-CT on 0, 7 and 14 days, and the number of osteoclasts was quantified by tartrate-resistant acid phosphatase (TRAP) staining. Moreover, the expression levels of RANKL and OPG in the compression area were measured histomorphometrically.
RESULTS
The WT group exhibited the typical pattern of OTM, characterized by narrowed periodontal space and bone resorption on the compression area. In contrast, the accelerated tooth displacement, increased osteoclast number (P < 0.0001) and bone resorption on the compression area in CB2 group. Additionally, the expression of RANKL was significantly upregulated, while OPG showed low levels in the compression area of the CB2 group (P < 0.0001).
CONCLUSIONS
CB2 modulated OTM and bone remodeling through regulating osteoclast activity and RANKL/OPG balance.
背景
本研究旨在探讨 CB2 在正畸牙齿移动过程中对骨骼调节的影响。
方法
将 30 只雄性小鼠随机分为 2 组（每组 n=15）：野生型（WT）组和 CB2 敲除（CB2）组。通过在上颌第一磨牙和中切牙之间施加镍钛螺旋弹簧诱导正畸牙齿移动（OTM）。WT 组内有三个亚组（0、7 和 14 天）和 CB2 组（0、7 和 14 天）。0 天组不施加力。通过 micro-CT 在 0、7 和 14 天评估牙齿移位、牙槽骨量和牙槽骨体积，并通过抗酒石酸酸性磷酸酶（TRAP）染色定量破骨细胞数量。此外，通过组织形态计量学测量压缩区中 RANKL 和 OPG 的表达水平。
结果
WT 组表现出典型的 OTM 模式，表现为牙周间隙变窄和压缩区骨吸收。相比之下，CB2 组的牙齿移动加速、破骨细胞数量增加（P<0.0001）和压缩区骨吸收增加。此外，CB2 组压缩区 RANKL 的表达显著上调，而 OPG 水平较低（P<0.0001）。
结论
CB2 通过调节破骨细胞活性和 RANKL/OPG 平衡来调节 OTM 和骨重塑。
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