Oral Disease Research Key Laboratory of Guizhou Tertiary Institution, Institute of Life Sciences, Zunyi Medical University, Zunyi, Guizhou, 563000, China.
Hospital of Stomatology, Zunyi Medical University, Zunyi, Guizhou, 563000, China.
Comb Chem High Throughput Screen. 2024;27(16):2350-2365. doi: 10.2174/0113862073256803231114095626.
The removal of impacted third molars by surgery may occur with a series of complications, whereas limited information about the postoperative pathogenesis is available. The objective of this study is to identify changes in gene expression after flap surgical removal of impacted third molars and provide potential information to reduce postoperative complications.
The gingival tissues of twenty patients with flap surgical removal of impacted third molars and twenty healthy volunteers were collected for gene expression testing. The collected gingival tissues were used RNA sequencing technology and quantitative real-time PCR validation was performed. DEG was mapped to protein databases such as GO and KEGG for functional annotation and, based on annotation information, for mining of differential expression genes in patients with mpacted third molars.
A total of 555 genes were differentially expressed. Among the top up-regulated genes, , and were strongly associated with immune response and signal transduction. Among the top down-regulated genes, and were related to keratinocyte differentiation, , and were related to bone mineralization, UGT2B17 is associated with susceptibility to osteoporosis. KEGG results showed that the DEGs were related to multiple disease-related pathways.
This first transcriptome analysis of gingival tissues from patients with surgical removal of impacted third molars provides new insights into postoperative genetic changes. The results may establish a basis for future research on minimizing the incidence of complications after flap-treated third molars.
手术拔除阻生第三磨牙可能会发生一系列并发症，而术后发病机制的相关信息有限。本研究旨在鉴定第三磨牙阻生经翻瓣手术后基因表达的变化，为减少术后并发症提供潜在信息。
收集 20 例接受翻瓣手术拔除阻生第三磨牙患者和 20 例健康志愿者的牙龈组织进行基因表达检测。采用 RNA 测序技术，对所采集的牙龈组织进行检测，通过定量实时 PCR 进行验证。DEG 被映射到 GO 和 KEGG 等蛋白质数据库进行功能注释，并基于注释信息挖掘阻生第三磨牙患者差异表达基因。
共发现 555 个差异表达基因。上调基因中， 、 和 与免疫反应和信号转导密切相关。下调基因中， 、 与角蛋白细胞分化有关， 、 与骨矿化有关，UGT2B17 与骨质疏松易感性有关。KEGG 结果显示，差异表达基因与多种疾病相关通路有关。
这是首次对接受手术拔除阻生第三磨牙患者的牙龈组织进行转录组分析，为术后遗传变化提供了新的见解。该结果可能为今后研究减少翻瓣治疗第三磨牙后并发症的发生率奠定基础。
