接骨木茎分泌的安息香的嫁接对化学成分、毒理学特性、抗血栓活性和心肌梗死保护作用的影响
Effects of grafting on chemical constituents, toxicological properties, antithrombotic activity, and myocardial infarction protection of styrax secreted from the trunk of Liquidambar orientalis Mill.
机构信息
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
出版信息
PLoS One. 2024 Jan 5;19(1):e0289894. doi: 10.1371/journal.pone.0289894. eCollection 2024.
Styrax, the balsam refined from the trunk of Liquidambar orientalis Mill. has a variety of applications in the perfumery and medical industry, especially for use in traditional Chinese medicine. However, the resources of styrax are in shortage due to being endangered of this plant. Grafting can improve the adaptability of plants to unfavorable environmental conditions. We tried to graft the L. orientalis Mill. on L. formosana Hance which was widely distributed in Jiangsu and Zhejiang provinces of China in an attempt to obtain styrax from grafted L. orientalis Mill. (grafted styrax, SG). Whether SG can become an alternative application of commercially available styrax (SC) need be further investigated. The components of SG were analyzed by GC-MS, and the results showed that the chromatograms of SG, SC, and styrax standard (SS) were consistent. The ration of 12 major chemical components based peak area in SG, SC, and SS were 93.95%, 94.24%, and 95.86% respectively. The assessment of toxicity, antithrombotic activity, and myocardial infarction protection of SG and SC was evaluated by using the zebrafish model, the results showed that SG and SC have the similar toxicological properties as evidenced by acute toxicity test, developmental toxicity and teratogenicity, and long-term toxicity test. Both SG and SC significantly decreased the thrombosis and increased blood flow velocity of zebrafish induced by adrenaline hydrochloride, inhibited myocardial apoptosis, myocardial infarction and myocardial inflammation in zebrafish induced by isoproterenol hydrochloride. Moreover, SG had an obvious improvement effect on cardiac output, while SC has no effect. Collectively, SG is similar to SC in chemical composition, toxicological properties, antithrombotic activity, and myocardial infarction protection effects, and may be used as a substitute for styrax to reduce the collection for wild L. orientalis Mill. and increase the available styrax resources.
苏合香，是从枫香树（Liquidambar orientalis Mill.）树干中精制而成的香脂，在香水和医疗行业有多种应用，尤其在中药方面。然而，由于该植物濒危，苏合香的资源短缺。嫁接可以提高植物对不利环境条件的适应性。我们尝试将枫香树（L. orientalis Mill.）嫁接到在中国江苏和浙江广泛分布的枫香树（L. formosana Hance）上，试图从嫁接的枫香树（接枝苏合香，SG）中获得苏合香。SG 是否可以成为市售苏合香（SC）的替代应用，还需要进一步研究。通过 GC-MS 对 SG 的成分进行了分析，结果表明 SG、SC 和苏合香标准品（SS）的色谱图一致。SG、SC 和 SS 中 12 种主要化学成分的峰面积比值分别为 93.95%、94.24%和 95.86%。通过斑马鱼模型评估了 SG 和 SC 的毒性、抗血栓活性和心肌梗死保护作用，结果表明，SG 和 SC 的急性毒性试验、发育毒性和致畸性以及长期毒性试验均显示出相似的毒理学特性。SG 和 SC 均显著降低了盐酸肾上腺素诱导的斑马鱼血栓形成和血流速度，抑制了盐酸异丙肾上腺素诱导的斑马鱼心肌细胞凋亡、心肌梗死和心肌炎症。此外，SG 对心输出量有明显改善作用，而 SC 则没有。综上所述，SG 在化学成分、毒理学特性、抗血栓活性和心肌梗死保护作用方面与 SC 相似，可作为苏合香的替代品，减少对野生枫香树的采集，增加苏合香的可用资源。
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